| pubmed-article:7948096 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7948096 | lifeskim:mentions | umls-concept:C1521827 | lld:lifeskim |
| pubmed-article:7948096 | lifeskim:mentions | umls-concept:C0208935 | lld:lifeskim |
| pubmed-article:7948096 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:7948096 | pubmed:dateCreated | 1994-12-23 | lld:pubmed |
| pubmed-article:7948096 | pubmed:abstractText | Asialoorosomucoid-polylysine (ASOR-PL) conjugates have been recently developed as carriers of electrostatically bound DNA for targeted delivery to the hepatic asialoglycoprotein receptor (ASGPr) for gene therapy. Using acid-urea gel electrophoresis we have found that previously reported procedures for the fractionation of ASOR-PL conjugates do not efficiently remove noncovalently bound polylysine (PL) from ASOR-PL. DNA complexes prepared with these conjugates have low solubilities, which limits their usefulness for subsequent experimentation, particularly in vivo. For ASOR-PL made by carbodiimide-mediated crosslinking with 5-kDa PL, dialysis against 1 M guanidine hydrochloride is effective to remove the low molecular weight unbound PL. Dialysis is not feasible when using higher molecular weight PLs, but preparative elution acid-urea gel electrophoresis was used to isolate crude ASOR-PL fractions free of unbound PL. ASOR-PL freed of PL by dialysis or electrophoresis was further fractionated by cation-exchange HPLC on carboxymethyl-functionalized columns eluted with a mixed pH-salt gradient. Early-eluting ASOR-PL fractions isolated by a combination of preparative elution acid-urea gel electrophoresis and cation-exchange HPLC were found to be preferred for the formation of soluble DNA complexes. | lld:pubmed |
| pubmed-article:7948096 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7948096 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7948096 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7948096 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7948096 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7948096 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7948096 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7948096 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7948096 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7948096 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7948096 | pubmed:issn | 1043-1802 | lld:pubmed |
| pubmed-article:7948096 | pubmed:author | pubmed-author:BeyHH | lld:pubmed |
| pubmed-article:7948096 | pubmed:author | pubmed-author:DeRomeM EME | lld:pubmed |
| pubmed-article:7948096 | pubmed:author | pubmed-author:FindeisM AMA | lld:pubmed |
| pubmed-article:7948096 | pubmed:author | pubmed-author:McKayT LTL | lld:pubmed |
| pubmed-article:7948096 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7948096 | pubmed:volume | 5 | lld:pubmed |
| pubmed-article:7948096 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7948096 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7948096 | pubmed:pagination | 306-11 | lld:pubmed |
| pubmed-article:7948096 | pubmed:dateRevised | 2004-11-17 | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:meshHeading | pubmed-meshheading:7948096-... | lld:pubmed |
| pubmed-article:7948096 | pubmed:articleTitle | Preparation of asialoorosomucoid-polylysine conjugates. | lld:pubmed |
| pubmed-article:7948096 | pubmed:affiliation | TargeTech, Inc., Meriden, Connecticut 06450. | lld:pubmed |
| pubmed-article:7948096 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7948096 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7948096 | lld:pubmed |
