| pubmed-article:7947799 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7947799 | lifeskim:mentions | umls-concept:C0036226 | lld:lifeskim |
| pubmed-article:7947799 | lifeskim:mentions | umls-concept:C0018549 | lld:lifeskim |
| pubmed-article:7947799 | lifeskim:mentions | umls-concept:C0542341 | lld:lifeskim |
| pubmed-article:7947799 | lifeskim:mentions | umls-concept:C1442792 | lld:lifeskim |
| pubmed-article:7947799 | lifeskim:mentions | umls-concept:C0056801 | lld:lifeskim |
| pubmed-article:7947799 | pubmed:issue | 46 | lld:pubmed |
| pubmed-article:7947799 | pubmed:dateCreated | 1994-12-21 | lld:pubmed |
| pubmed-article:7947799 | pubmed:abstractText | We have studied the effects of cyclopiazonic acid (CPA) and halothane on the enzymatic activity, oligomeric state, and conformational equilibrium of the Ca-ATPase in skeletal muscle sarcoplasmic reticulum (SR). CPA is a potent inhibitor of Ca-ATPase activity, and this inhibition is competitive with respect to ATP concentration. Time-resolved phosphorescence anisotropy was used to detect the fraction of Ca-ATPase monomers, dimers, and larger aggregates in the absence and presence of CPA. CPA increased the fraction of dimers and larger aggregates of the Ca-ATPase. Addition of halothane to SR, or detergent solubilization of the Ca-ATPase, increased the apparent KI of CPA inhibition, and increased the fraction of Ca-ATPase present as monomers. CPA stabilized the E2 conformational state of the Ca-ATPase relative to the E1 and E2-P states, as measured by fluorescein 5-isothiocyanate fluorescence and enzyme phosphorylation from inorganic phosphate. E2-P formation in the presence of CPA was partially restored by halothane and solubilization. We conclude that CPA inhibits the Ca-ATPase in part by overstabilizing dimers or small oligomers of the Ca-ATPase, which is correlated with stabilization of the E2 conformation of the enzyme. | lld:pubmed |
| pubmed-article:7947799 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7947799 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7947799 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7947799 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7947799 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7947799 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7947799 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7947799 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7947799 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7947799 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7947799 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:7947799 | pubmed:author | pubmed-author:ThomasD DDD | lld:pubmed |
| pubmed-article:7947799 | pubmed:author | pubmed-author:MahaneyJ EJE | lld:pubmed |
| pubmed-article:7947799 | pubmed:author | pubmed-author:KaronB SBS | lld:pubmed |
| pubmed-article:7947799 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7947799 | pubmed:day | 22 | lld:pubmed |
| pubmed-article:7947799 | pubmed:volume | 33 | lld:pubmed |
| pubmed-article:7947799 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7947799 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7947799 | pubmed:pagination | 13928-37 | lld:pubmed |
| pubmed-article:7947799 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
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| pubmed-article:7947799 | pubmed:meshHeading | pubmed-meshheading:7947799-... | lld:pubmed |
| pubmed-article:7947799 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7947799 | pubmed:articleTitle | Halothane and cyclopiazonic acid modulate Ca-ATPase oligomeric state and function in sarcoplasmic reticulum. | lld:pubmed |
| pubmed-article:7947799 | pubmed:affiliation | Department of Biochemistry, University of Minnesota Medical School, Minneapolis 55455. | lld:pubmed |
| pubmed-article:7947799 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7947799 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7947799 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7947799 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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