| pubmed-article:7945913 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7945913 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7945913 | lifeskim:mentions | umls-concept:C1328893 | lld:lifeskim |
| pubmed-article:7945913 | lifeskim:mentions | umls-concept:C0225336 | lld:lifeskim |
| pubmed-article:7945913 | lifeskim:mentions | umls-concept:C0026046 | lld:lifeskim |
| pubmed-article:7945913 | lifeskim:mentions | umls-concept:C1261381 | lld:lifeskim |
| pubmed-article:7945913 | lifeskim:mentions | umls-concept:C0238815 | lld:lifeskim |
| pubmed-article:7945913 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7945913 | pubmed:dateCreated | 1994-12-6 | lld:pubmed |
| pubmed-article:7945913 | pubmed:abstractText | When grown in the presence of Matrigel, monolayers of an immortalized human microvascular cell line (HMEC-1) form capillary-like microtubule networks. Previous work, using HMEC-1 monolayers, demonstrated a significant difference in in vitro cytotoxicity between Brazilian purpuric fever (BPF)-associated Haemophilus influenzae biogroup aegyptius (HAE) strains and non-BPF-associated HAE strains. The present study demonstrates that BPF-related cytotoxic differences can also be observed in HMEC-1 microtubule networks. At a multiplicity of infection (MOI) of 2 x 10(-2) bacteria/tissue culture cell, BPF-associated strain F3031 disrupted the microtubule network, producing random clumps of rounded cells at 48 h of incubation. Infection with non-BPF-associated strain F1947 at the same MOI produced no observable microtubule disruption. The ability of HMEC-1 microtubule model to differentiate virulent and avirulent HAE in vitro will further aid in the study of BPF pathogenesis. In addition, the fact that the HMEC-1 cells can be induced to form microtubules make it an excellent model system for the general study of many of the agents of vascular purpura. | lld:pubmed |
| pubmed-article:7945913 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7945913 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7945913 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7945913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7945913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7945913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7945913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7945913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7945913 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7945913 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7945913 | pubmed:issn | 1015-2008 | lld:pubmed |
| pubmed-article:7945913 | pubmed:author | pubmed-author:AdesE WEW | lld:pubmed |
| pubmed-article:7945913 | pubmed:author | pubmed-author:CandalF JFJ | lld:pubmed |
| pubmed-article:7945913 | pubmed:author | pubmed-author:QuinnF DFD | lld:pubmed |
| pubmed-article:7945913 | pubmed:author | pubmed-author:WeyantR SRS | lld:pubmed |
| pubmed-article:7945913 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7945913 | pubmed:volume | 62 | lld:pubmed |
| pubmed-article:7945913 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7945913 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7945913 | pubmed:pagination | 109-12 | lld:pubmed |
| pubmed-article:7945913 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:meshHeading | pubmed-meshheading:7945913-... | lld:pubmed |
| pubmed-article:7945913 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7945913 | pubmed:articleTitle | Destruction of human microvascular endothelial cell capillary-like microtubules by Brazilian purpuric fever-associated Haemophilus influenzae biogroup aegyptius. | lld:pubmed |
| pubmed-article:7945913 | pubmed:affiliation | Emerging Bacterial and Mycotic Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Ga 30333. | lld:pubmed |
| pubmed-article:7945913 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7945913 | pubmed:publicationType | Comparative Study | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7945913 | lld:pubmed |
