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pubmed-article:7943633pubmed:abstractTextAmplification of viral DNA offers a potentially sensitive and specific method for identifying herpes viruses in pathologic specimens. The purpose of this study is to assess the value of polymerase chain reaction amplification of DNA as a diagnostic test for herpes virus in pathologic specimens. The purpose of this study is to assess for herpes virus infections. We examined 79 paraffin-embedded tissue samples from 43 patients and 55 viral culture samples from 45 patients. Herpes virus DNA in the specimens was amplified by polymerase chain reaction. Using paraffin-embedded tissue on which a diagnosis of herpes virus was made by morphologic criteria, 11 of 19 patients had herpes virus DNA identified by PCR; herpes virus DNA was not identified in any of 35 negative control specimens. Herpes virus DNA was also identified by polymerase chain reaction in all of the positive herpes virus culture specimens. Of 29 culture negative specimens, herpes virus DNA was identified in six. We conclude that polymerase chain reaction is useful to establish or confirm the presence of a herpes virus infection in paraffin-embedded tissue samples and that it is more sensitive than viral culture.lld:pubmed
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pubmed-article:7943633pubmed:authorpubmed-author:SmithS ESElld:pubmed
pubmed-article:7943633pubmed:authorpubmed-author:WhiteW LWLlld:pubmed
pubmed-article:7943633pubmed:authorpubmed-author:ThomasC ACAlld:pubmed
pubmed-article:7943633pubmed:authorpubmed-author:MorganT MTMlld:pubmed
pubmed-article:7943633pubmed:authorpubmed-author:FeldmanS RSRlld:pubmed
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pubmed-article:7943633pubmed:volume16lld:pubmed
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pubmed-article:7943633pubmed:pagination268-74lld:pubmed
pubmed-article:7943633pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:7943633pubmed:year1994lld:pubmed
pubmed-article:7943633pubmed:articleTitleClinical application of polymerase chain reaction amplification to diagnosis of herpes virus infection.lld:pubmed
pubmed-article:7943633pubmed:affiliationDepartment of Dermatology, Bowman Gray School of Medicine, Wake Forest University, Winston-Salem, North Carolina.lld:pubmed
pubmed-article:7943633pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7943633pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:7943633pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed