| pubmed-article:7931572 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7931572 | lifeskim:mentions | umls-concept:C0027882 | lld:lifeskim |
| pubmed-article:7931572 | lifeskim:mentions | umls-concept:C0020663 | lld:lifeskim |
| pubmed-article:7931572 | lifeskim:mentions | umls-concept:C0014912 | lld:lifeskim |
| pubmed-article:7931572 | lifeskim:mentions | umls-concept:C0332161 | lld:lifeskim |
| pubmed-article:7931572 | lifeskim:mentions | umls-concept:C1637379 | lld:lifeskim |
| pubmed-article:7931572 | lifeskim:mentions | umls-concept:C0205130 | lld:lifeskim |
| pubmed-article:7931572 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:7931572 | pubmed:dateCreated | 1994-11-14 | lld:pubmed |
| pubmed-article:7931572 | pubmed:abstractText | The mu-opioid agonist DAMGO (Tyr-D-Ala-Gly-MePhe-Gly-ol) hyperpolarizes the majority of arcuate hypothalamic (ARC) neurons by opening an inwardly rectifying potassium conductance. The EC50 for the DAMGO-induced hyperpolarization was 60 +/- 3 nM in ARC neurons from ovariectomized guinea pigs. Superfusion of 17 beta-estradiol (E2; 100 nM) for 20 min in vitro resulted in a significant decrease in DAMGO potency (EC50 = 212 +/- 16 nM) in 40% of the neurons that were tested. This rapid effect of E2 on the mu-opioid response was not mimicked by the biologically inactive isomer 17 alpha-estradiol. Multiple concentrations of E2 were used to generate an E2 concentration-response curve, with an EC50 of 9 nM and a maximal increase in the DAMGO EX50 of 411% of controls. The membrane properties and firing rate of E2-sensitive and E2-insensitive neurons were not different. Streptavidin-FITC labeling did not reveal any significant morphological differences between the groups, but a higher number of E2-sensitive cells was found in the lateral ARC and cell-poor zone. Moreover, immunocytochemical staining of the recorded cells revealed that beta-endorphin neurons were among those sensitive to E2. Therefore, E2 could increase beta-endorphin release by decreasing the potency of beta-endorphinergic autoinhibition, thus increasing the tonic opioid inhibition of E2-insensitive cells. Furthermore, the diffuse projections of hypothalamic beta-endorphin neurons would allow E2 to alter processes throughout the brain, as well as having local effects in the hypothalamus. | lld:pubmed |
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| pubmed-article:7931572 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7931572 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7931572 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7931572 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7931572 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7931572 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7931572 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7931572 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7931572 | pubmed:issn | 0270-6474 | lld:pubmed |
| pubmed-article:7931572 | pubmed:author | pubmed-author:KellyM JMJ | lld:pubmed |
| pubmed-article:7931572 | pubmed:author | pubmed-author:RonnekleivO... | lld:pubmed |
| pubmed-article:7931572 | pubmed:author | pubmed-author:LagrangeA HAH | lld:pubmed |
| pubmed-article:7931572 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7931572 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:7931572 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7931572 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7931572 | pubmed:pagination | 6196-204 | lld:pubmed |
| pubmed-article:7931572 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
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| pubmed-article:7931572 | pubmed:meshHeading | pubmed-meshheading:7931572-... | lld:pubmed |
| pubmed-article:7931572 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7931572 | pubmed:articleTitle | The potency of mu-opioid hyperpolarization of hypothalamic arcuate neurons is rapidly attenuated by 17 beta-estradiol. | lld:pubmed |
| pubmed-article:7931572 | pubmed:affiliation | Department of Physiology, Oregon Health Sciences University, Portland 97201-3098. | lld:pubmed |
| pubmed-article:7931572 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7931572 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7931572 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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