| pubmed-article:7929767 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7929767 | lifeskim:mentions | umls-concept:C0024198 | lld:lifeskim |
| pubmed-article:7929767 | lifeskim:mentions | umls-concept:C0006034 | lld:lifeskim |
| pubmed-article:7929767 | lifeskim:mentions | umls-concept:C0600324 | lld:lifeskim |
| pubmed-article:7929767 | lifeskim:mentions | umls-concept:C1524063 | lld:lifeskim |
| pubmed-article:7929767 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:7929767 | pubmed:dateCreated | 1994-11-21 | lld:pubmed |
| pubmed-article:7929767 | pubmed:abstractText | Infection with Borrelia burgdorferi, the etiologic agent of Lyme disease, is associated with an early and dominant humoral response to the spirochete's 23-kDa outer surface protein C (OspC). We have cloned and expressed OspC as a fusion protein in Escherichia coli and have shown that patient serum samples react with it in an enzyme-linked immunosorbent assay (ELISA) (S. J. Padula, A. Sampieri, F. Dias, A. Szczepanski, and R. W. Ryan, Infect. Immun. 61:5097-5105, 1993). Now we have compared the detection of B. burgdorferi-specific immunoglobulin M antibodies in 74 individuals with culture-positive erythema migrans by a whole-cell ELISA, immunoblot, and the recombinant OspC (rOspC) ELISA. Seventy-six negative controls were also studied. With all of the tests, there was a statistically significant association between the duration of disease and the frequency of a positive result. With the rOspC ELISA, the predictive value of a positive test was 100% and the predictive value of a negative test was 74%. Similar results were obtained with the whole-cell ELISA and with the immunoblot using as the source of test antigen a strain of B. burgdorferi which expresses abundant levels of OspC. We conclude that the use of rOspC in an ELISA is a convenient, readily automated, and easily standardized test for the serodiagnosis of early Lyme disease. | lld:pubmed |
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| pubmed-article:7929767 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7929767 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7929767 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7929767 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7929767 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:7929767 | pubmed:issn | 0095-1137 | lld:pubmed |
| pubmed-article:7929767 | pubmed:author | pubmed-author:RyanR WRW | lld:pubmed |
| pubmed-article:7929767 | pubmed:author | pubmed-author:CravenR BRB | lld:pubmed |
| pubmed-article:7929767 | pubmed:author | pubmed-author:DiasFF | lld:pubmed |
| pubmed-article:7929767 | pubmed:author | pubmed-author:PadulaS JSJ | lld:pubmed |
| pubmed-article:7929767 | pubmed:author | pubmed-author:SampieriAA | lld:pubmed |
| pubmed-article:7929767 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7929767 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:7929767 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7929767 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7929767 | pubmed:pagination | 1733-8 | lld:pubmed |
| pubmed-article:7929767 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7929767 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7929767 | pubmed:articleTitle | Use of recombinant OspC from Borrelia burgdorferi for serodiagnosis of early Lyme disease. | lld:pubmed |
| pubmed-article:7929767 | pubmed:affiliation | Department of Medicine, University of Connecticut Health Center, Farmington 06030. | lld:pubmed |
| pubmed-article:7929767 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7929767 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7929767 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7929767 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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