| pubmed-article:7915202 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0023859 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0004561 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0039194 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0205160 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C1446409 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C1332714 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0205369 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:7915202 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:7915202 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7915202 | pubmed:dateCreated | 1994-9-27 | lld:pubmed |
| pubmed-article:7915202 | pubmed:abstractText | We have reported that Listeria monocytogenes-specific IFN-gamma-producing CD4+ T cells are induced by in vitro 5-day culture of naive spleen cells with viable L. monocytogenes (VLM), but not induced by culture with heat-killed L. monocytogenes (HKLM). In the present study, the role of B cells in the regulation of induction of IFN-gamma-producing CD4+ T cells in the in vitro system was investigated. We found that L. monocytogenes-specific IFN-gamma-producing CD4+ T cells were not generated when B cells were depleted from spleen cells before culture with VLM although Ag-specific proliferative response was retained. IFN-gamma production by CD4+ T cells was restored by addition of B cells cultured with VLM to the culture of B cell-depleted spleen cells and VLM. In contrast, B cells cultured with HKLM could not restore the induction of IFN-gamma production when added in culture of B cell-depleted spleen cells and VLM. Analysis of cytokine gene expression by reverse transcription-polymerase chain reaction method revealed that expression of interleukin-10 (IL-10) was higher but TNF-alpha was lower in B cells cultured with HKLM when compared with that in B cells cultured with VLM. Furthermore, addition of neutralizing anti-IL-10 mAb into culture of naive spleen cells with HKLM resulted in appearance of IFN-gamma-producing cells. These results suggest that B cells have positive and negative roles in the induction of IFN-gamma-producing CD4+ T cells. The inhibition of induction of IFN-gamma-producing CD4+ T cells may depend on B cell-derived IL-10. | lld:pubmed |
| pubmed-article:7915202 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7915202 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7915202 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7915202 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7915202 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7915202 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7915202 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7915202 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7915202 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7915202 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7915202 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:7915202 | pubmed:issn | 0008-8749 | lld:pubmed |
| pubmed-article:7915202 | pubmed:author | pubmed-author:NomotoKK | lld:pubmed |
| pubmed-article:7915202 | pubmed:author | pubmed-author:MitsuyamaMM | lld:pubmed |
| pubmed-article:7915202 | pubmed:author | pubmed-author:SonoNN | lld:pubmed |
| pubmed-article:7915202 | pubmed:author | pubmed-author:MatsuzakiGG | lld:pubmed |
| pubmed-article:7915202 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7915202 | pubmed:volume | 157 | lld:pubmed |
| pubmed-article:7915202 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7915202 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7915202 | pubmed:pagination | 403-14 | lld:pubmed |
| pubmed-article:7915202 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:7915202 | pubmed:meshHeading | pubmed-meshheading:7915202-... | lld:pubmed |
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| pubmed-article:7915202 | pubmed:meshHeading | pubmed-meshheading:7915202-... | lld:pubmed |
| pubmed-article:7915202 | pubmed:meshHeading | pubmed-meshheading:7915202-... | lld:pubmed |
| pubmed-article:7915202 | pubmed:meshHeading | pubmed-meshheading:7915202-... | lld:pubmed |
| pubmed-article:7915202 | pubmed:meshHeading | pubmed-meshheading:7915202-... | lld:pubmed |
| pubmed-article:7915202 | pubmed:meshHeading | pubmed-meshheading:7915202-... | lld:pubmed |
| pubmed-article:7915202 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7915202 | pubmed:articleTitle | The role of B cells in in vitro induction of IFN-gamma-producing CD4+ T cells specific to Listeria monocytogens: positive and IL-10-mediated negative regulation. | lld:pubmed |
| pubmed-article:7915202 | pubmed:affiliation | Department of Immunology, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan. | lld:pubmed |
| pubmed-article:7915202 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7915202 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7915202 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7915202 | lld:pubmed |
