| pubmed-article:7913440 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7913440 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7913440 | lifeskim:mentions | umls-concept:C0032005 | lld:lifeskim |
| pubmed-article:7913440 | lifeskim:mentions | umls-concept:C0815000 | lld:lifeskim |
| pubmed-article:7913440 | lifeskim:mentions | umls-concept:C1305923 | lld:lifeskim |
| pubmed-article:7913440 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:7913440 | lifeskim:mentions | umls-concept:C2349975 | lld:lifeskim |
| pubmed-article:7913440 | lifeskim:mentions | umls-concept:C0070750 | lld:lifeskim |
| pubmed-article:7913440 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7913440 | pubmed:dateCreated | 1994-8-17 | lld:pubmed |
| pubmed-article:7913440 | pubmed:abstractText | We investigated a regulatory mechanism for the production of pituitary adenylate-cyclase-activating polypeptide (PACAP) using the human neuroblastoma cell line, IMR-32. Although immunoreactive (ir-) PACAP38 was produced by these cells, it was not accumulated in the culture medium. To overcome this problem, we cultured the cells in the presence of a biotinylated monoclonal antibody to PACAP38 in order to trap ir-PACAP38 in the medium. Then, the trapped ir-PACAP38 was measured with a two-site enzyme immunoassay based on the biotin-avidin interaction. These studies showed that confluent IMR-32 cells constitutively secreted at least 130 pg/10(6) cells per day of ir-PACAP38. Treatment of the cells with 1 mM 1, N6-dibutyryladenosine 3',5'-monophosphate (Bt2cAMP) or 0.1 microM phorbol 12-myristate 13-acetate stimulated ir-PACAP38 production at almost twice the basal level in IMR-32 cells, and these two reagents additively stimulated ir-PACAP38 production. Northern blot analysis indicated that treatment of the cells with both Bt2cAMP and the phorbol ester increased the intensity of several mRNA bands that hybridized with a cDNA probe for human PACAP precursor. We also found that IMR-32 cells possessed high-affinity type-I PACAP receptors (Kd = 230 pM, binding sites: 8.6 x 10(4) sites/cell), which had a 1000-fold higher affinity for PACAP38 and PACAP27 than for vasoactive intestinal polypeptide. The treatment of IMR-32 cells with PACAP and vasoactive intestinal polypeptide increased intracellular cAMP levels, and also increased ir-PACAP production. Northern blot analysis revealed that PACAP at 10 nM markedly stimulated the expression of a 3.7-kb mRNA band that hybridized with PACAP precursor cDNA. These results indicate that IMR-32 cells produce ir-PACAP38, and that PACAP stimulates the synthesis of PACAP mRNA and peptides by increasing intracellular cAMP. Although it remains to be investigated whether PACAP secreted by IMR-32 cells really acts in an autocrine fashion, the present study provides a useful model for further studies of the biological role of PACAP in neuronal cells that produce PACAP and also respond to PACAP. | lld:pubmed |
| pubmed-article:7913440 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7913440 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7913440 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7913440 | pubmed:month | Jul | lld:pubmed |
| pubmed-article:7913440 | pubmed:issn | 0014-2956 | lld:pubmed |
| pubmed-article:7913440 | pubmed:author | pubmed-author:HaradaMM | lld:pubmed |
| pubmed-article:7913440 | pubmed:author | pubmed-author:SuzukiNN | lld:pubmed |
| pubmed-article:7913440 | pubmed:author | pubmed-author:FujinoMM | lld:pubmed |
| pubmed-article:7913440 | pubmed:author | pubmed-author:HosoyaMM | lld:pubmed |
| pubmed-article:7913440 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7913440 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:7913440 | pubmed:volume | 223 | lld:pubmed |
| pubmed-article:7913440 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7913440 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7913440 | pubmed:pagination | 147-53 | lld:pubmed |
| pubmed-article:7913440 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
| pubmed-article:7913440 | pubmed:meshHeading | pubmed-meshheading:7913440-... | lld:pubmed |
| pubmed-article:7913440 | pubmed:meshHeading | pubmed-meshheading:7913440-... | lld:pubmed |
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| pubmed-article:7913440 | pubmed:meshHeading | pubmed-meshheading:7913440-... | lld:pubmed |
| pubmed-article:7913440 | pubmed:meshHeading | pubmed-meshheading:7913440-... | lld:pubmed |
| pubmed-article:7913440 | pubmed:meshHeading | pubmed-meshheading:7913440-... | lld:pubmed |
| pubmed-article:7913440 | pubmed:meshHeading | pubmed-meshheading:7913440-... | lld:pubmed |
| pubmed-article:7913440 | pubmed:meshHeading | pubmed-meshheading:7913440-... | lld:pubmed |
| pubmed-article:7913440 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7913440 | pubmed:articleTitle | Enhanced production of pituitary adenylate-cyclase-activating polypeptide by 1, N6-dibutyryladenosine 3',5'-monophosphate, phorbol 12-myristate 13-acetate and by the polypeptide itself in human neuroblastoma cells, IMR-32. | lld:pubmed |
| pubmed-article:7913440 | pubmed:affiliation | Discovery Research Division, Takeda Chemical Industries Ltd, Ibaraki, Japan. | lld:pubmed |
| pubmed-article:7913440 | pubmed:publicationType | Journal Article | lld:pubmed |
