| pubmed-article:7905499 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0021756 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0242633 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0080202 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C2362651 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0030943 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0040648 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C1423842 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0443199 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C2249859 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C1157563 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0441712 | lld:lifeskim |
| pubmed-article:7905499 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:7905499 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7905499 | pubmed:dateCreated | 1994-3-9 | lld:pubmed |
| pubmed-article:7905499 | pubmed:abstractText | Regulation of IL-2 gene expression in response to receptor-mediated stimuli is known to be mediated primarily by the IL-2 transcriptional enhancer and multiple transcription factors. However, the mechanism that controls the differential expression of the IL-2 gene in both human and murine CD4+ Th cell subsets (Th1-IL-2+ and Th2-IL-2-) is not clearly understood. Differential IL-2 gene expression was assessed in murine Th1 and Th2 subsets by analyzing the expression of a Escherichia coli lacZ reporter gene under control of the human IL-2 enhancer (IL2ZH) transfected in both T cell subsets. Stimulation of transfected T cells with the mitogen Con A, anti-CD3 Ab, or PMA plus ionomycin activated the IL2ZH construct in Th1 but not Th2 cells. However, IL2ZH was activated in stimulated Th2 cells that were co-transfected with a vector that overexpressed the eukaryotic initiation factor 4E (eIF-4E). It has been shown that eIF-4E is rate limiting for protein synthesis and its overexpression leads to increased rates of protein synthesis. Hence, eIF-4E overexpression could have overcome a deficiency in transcriptionally active levels of IL-2 regulatory factors in Th2 cells leading to IL-2 enhancer activation. This possibility was supported by demonstrating that transcriptionally active levels of the critical IL-2 transcription factor, nuclear factor of activated T cells (NF-AT), occurred only in Th2 cells overexpressing eIF-4E but not in normal Th2 cells, thus indicating that the inability of Th2 cells to express IL-2 was associated with inadequate levels of at least one transcription factor, NF-AT. Moreover, these results were confirmed by the observation that eIF-4E overexpression augmented NF-AT binding activity in Th2 cells. These data suggest that concentrations of inducible transcription factors are a major component of the regulatory mechanisms dictating IL-2 expression and may be under translational control in Th1/Th2 T cell subsets. | lld:pubmed |
| pubmed-article:7905499 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7905499 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7905499 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7905499 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7905499 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7905499 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:7905499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7905499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7905499 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7905499 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7905499 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:7905499 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:7905499 | pubmed:author | pubmed-author:KaplanA MAM | lld:pubmed |
| pubmed-article:7905499 | pubmed:author | pubmed-author:RhoadsR ERE | lld:pubmed |
| pubmed-article:7905499 | pubmed:author | pubmed-author:CowanW AWA | lld:pubmed |
| pubmed-article:7905499 | pubmed:author | pubmed-author:De... | lld:pubmed |
| pubmed-article:7905499 | pubmed:author | pubmed-author:BarveS SSS | lld:pubmed |
| pubmed-article:7905499 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7905499 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:7905499 | pubmed:volume | 152 | lld:pubmed |
| pubmed-article:7905499 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7905499 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7905499 | pubmed:pagination | 1171-81 | lld:pubmed |
| pubmed-article:7905499 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:7905499 | pubmed:meshHeading | pubmed-meshheading:7905499-... | lld:pubmed |
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| pubmed-article:7905499 | pubmed:meshHeading | pubmed-meshheading:7905499-... | lld:pubmed |
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| pubmed-article:7905499 | pubmed:meshHeading | pubmed-meshheading:7905499-... | lld:pubmed |
| pubmed-article:7905499 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7905499 | pubmed:articleTitle | Mechanism of differential regulation of IL-2 in murine Th1 and Th2 T cell subsets. 1. Induction of IL-2 transcription in Th2 cells by up-regulation of transcription factors with the protein synthesis initiation factor 4E. | lld:pubmed |
| pubmed-article:7905499 | pubmed:affiliation | Department of Microbiology and Immunology, University of Kentucky College of Medicine, Lexington 40536. | lld:pubmed |
| pubmed-article:7905499 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7905499 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7905499 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:16183 | entrezgene:pubmed | pubmed-article:7905499 | lld:entrezgene |
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