| pubmed-article:7901328 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7901328 | lifeskim:mentions | umls-concept:C0018038 | lld:lifeskim |
| pubmed-article:7901328 | lifeskim:mentions | umls-concept:C0006104 | lld:lifeskim |
| pubmed-article:7901328 | lifeskim:mentions | umls-concept:C0018328 | lld:lifeskim |
| pubmed-article:7901328 | lifeskim:mentions | umls-concept:C1704675 | lld:lifeskim |
| pubmed-article:7901328 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:7901328 | lifeskim:mentions | umls-concept:C0205812 | lld:lifeskim |
| pubmed-article:7901328 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:7901328 | pubmed:dateCreated | 1993-11-29 | lld:pubmed |
| pubmed-article:7901328 | pubmed:abstractText | Recent reports have suggested that a major proportion of [3H]kainate binding in goldfish brain is to a novel form of G-protein-linked glutamate receptor. Here we confirm that guanine nucleotides decrease [3H]kainate binding in goldfish brain membranes, but that binding is also reduced to a similar extent under conditions where G-protein modulation should be minimised. Inclusion of GTP gamma S resulted in an approximately twofold decrease in the affinity of [3H]kainate binding and a 50% reduction in the apparent Bmax values in both Mg2+/Na+ and Mg2+/Na(+)-free buffer when assayed at 0 degrees C. The pharmacology of [3H]kainate binding is similar to that of well-characterised ionotropic kainate receptors but unlike that of known metabotropic glutamate receptors, with neither 1S,3R-amino-1,3-cyclopentanedicarboxylic acid (1S,3R-ACPD) nor ibotenic acid being effective competitors. The molecular mass of the [3H]kainate binding protein, as determined by radiation inactivation, was 40 kDa, similar to the subunit sizes of other lower vertebrate kainate binding proteins that are believed to comprise ligand-gated ion channels. Furthermore, GTP gamma S also inhibited the binding of the non-NMDA receptor-selective antagonist 6-[3H]cyano-7-nitroquinoxaline-2,3-dione. These data strongly suggest that the regulatory interaction between guanine nucleotides and [3H]kainate and 6-[3H]cyano-7-nitroquinoxaline-2,3-dione binding is complex and involves competition at the agonist/antagonist binding site in addition to any G-protein-mediated modulation. | lld:pubmed |
| pubmed-article:7901328 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7901328 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7901328 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7901328 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7901328 | pubmed:issn | 0022-3042 | lld:pubmed |
| pubmed-article:7901328 | pubmed:author | pubmed-author:MurphyP APA | lld:pubmed |
| pubmed-article:7901328 | pubmed:author | pubmed-author:BarnesJ MJM | lld:pubmed |
| pubmed-article:7901328 | pubmed:author | pubmed-author:KirkhamDD | lld:pubmed |
| pubmed-article:7901328 | pubmed:author | pubmed-author:HenleyJ MJM | lld:pubmed |
| pubmed-article:7901328 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7901328 | pubmed:volume | 61 | lld:pubmed |
| pubmed-article:7901328 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7901328 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7901328 | pubmed:pagination | 1685-91 | lld:pubmed |
| pubmed-article:7901328 | pubmed:dateRevised | 2009-9-29 | lld:pubmed |
| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
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| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
| pubmed-article:7901328 | pubmed:meshHeading | pubmed-meshheading:7901328-... | lld:pubmed |
| pubmed-article:7901328 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:7901328 | pubmed:articleTitle | Interaction of guanine nucleotides with [3H]kainate and 6-[3H]cyano-7-nitroquinoxaline-2,3-dione binding in goldfish brain. | lld:pubmed |
| pubmed-article:7901328 | pubmed:affiliation | Department of Pharmacology, Medical School, University of Birmingham, England. | lld:pubmed |
| pubmed-article:7901328 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7901328 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
