| pubmed-article:7896711 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7896711 | lifeskim:mentions | umls-concept:C0085470 | lld:lifeskim |
| pubmed-article:7896711 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:7896711 | lifeskim:mentions | umls-concept:C0007009 | lld:lifeskim |
| pubmed-article:7896711 | lifeskim:mentions | umls-concept:C0038187 | lld:lifeskim |
| pubmed-article:7896711 | lifeskim:mentions | umls-concept:C0038952 | lld:lifeskim |
| pubmed-article:7896711 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7896711 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:7896711 | pubmed:dateCreated | 1995-4-26 | lld:pubmed |
| pubmed-article:7896711 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7896711 | pubmed:abstractText | By using mini-Tn5 transposon mutagenesis, two mutants of Pseudomonas putida ATCC 12633 were isolated which showed a marked increase in their sensitivity to carbon starvation; these mutants are presumably affected in the Pex type of proteins that P. putida induces upon carbon starvation (M. Givskov, L. Eberl, and S. Molin, J. Bacteriol. 176:4816-4824, 1994). The affected genes in our mutants were induced about threefold upon carbon starvation. The promoter region of the starvation gene in the mutant MK107 possessed a strong sigma 54-type-promoter sequence, and deletion analysis suggested that this was the major promoter regulating expression; this was confirmed by transcript mapping in rpoN+ and rpoN mutant backgrounds. The deletion analysis implicated a sequence upstream of the sigma 54 promoter, as well as a region downstream of the transcription start site, in the functioning of the promoter. Two sigma 70-type Pribnow boxes were also detected in the promoter region, but their transcriptional activity in the wild type was very weak. However, in a sigma 54-deficient background, these promoters became stronger. The mechanism and possible physiological role of this phenomenon and the possibility that the sequence upstream of the sigma 54 promoter may have a role in carbon sensing are discussed. | lld:pubmed |
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| pubmed-article:7896711 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7896711 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7896711 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7896711 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7896711 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:7896711 | pubmed:issn | 0021-9193 | lld:pubmed |
| pubmed-article:7896711 | pubmed:author | pubmed-author:MatinAA | lld:pubmed |
| pubmed-article:7896711 | pubmed:author | pubmed-author:KimYY | lld:pubmed |
| pubmed-article:7896711 | pubmed:author | pubmed-author:WatrudL SLS | lld:pubmed |
| pubmed-article:7896711 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7896711 | pubmed:volume | 177 | lld:pubmed |
| pubmed-article:7896711 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7896711 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7896711 | pubmed:pagination | 1850-9 | lld:pubmed |
| pubmed-article:7896711 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7896711 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7896711 | pubmed:articleTitle | A carbon starvation survival gene of Pseudomonas putida is regulated by sigma 54. | lld:pubmed |
| pubmed-article:7896711 | pubmed:affiliation | Department of Microbiology and Immunology, Stanford University School of Medicine, California 94305-5402. | lld:pubmed |
| pubmed-article:7896711 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7896711 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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