| pubmed-article:7890742 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7890742 | lifeskim:mentions | umls-concept:C2733653 | lld:lifeskim |
| pubmed-article:7890742 | lifeskim:mentions | umls-concept:C0678594 | lld:lifeskim |
| pubmed-article:7890742 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:7890742 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:7890742 | pubmed:dateCreated | 1995-4-18 | lld:pubmed |
| pubmed-article:7890742 | pubmed:abstractText | The CD52 antigen was extracted from human spleens with organic solvents and purified by immunoaffinity and reverse-phase chromatography. The latter step resolved two CD52 species, called CD52-I and CD52-II. Both species were found to contain similar N-linked oligosaccharides and glycosylphosphatidylinositol (GPI) anchor glycans. The N-linked oligosaccharides were characterized by methylation linkage analysis and, following exhaustive neuraminidase and endo-beta-galactosidase digestion, by the reagent array analysis method. The results showed that the single CD52 N-glycosylation site is occupied by large sialylated, polylactosamine-containing, core-fucosylated tetraantennary oligosaccharides. The locations of the phosphoryl substituents on the GPI anchor glycan were determined using a new and sensitive method based upon partial acid hydrolysis of the GPI glycan. The difference between CD52-I and CD52-II was in the phosphatidylinositol (PI) moieties of the GPI anchors. The phosphatidylinositol-specific phospholipase C-sensitive CD52-I contained exclusively distearoyl-PI, while the PI-phospholipase C-resistant CD52-II contained predominantly a palmitoylated stearoyl-arachidonoyl-PI, as judged by electrospray ionization mass spectrometry. Tandem mass spectrometric studies indicated that the palmitoyl residue of the CD52-II anchor is attached to the 2-position of the myo-inositol ring. Both the CD52-I and CD52-II PI structures are unusual for GPI anchors and the possible significance of this is discussed. The alkali-lability of the CD52 epitope recognized by the Campath-1H monoclonal antibody was studied. The data suggest that the alkali-labile hydroxyester-linked fatty acids of the GPI anchor are necessary for antibody binding. | lld:pubmed |
| pubmed-article:7890742 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7890742 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890742 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7890742 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7890742 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7890742 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:7890742 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:7890742 | pubmed:author | pubmed-author:SchneiderPP | lld:pubmed |
| pubmed-article:7890742 | pubmed:author | pubmed-author:FergusonM AMA | lld:pubmed |
| pubmed-article:7890742 | pubmed:author | pubmed-author:LifelyM RMR | lld:pubmed |
| pubmed-article:7890742 | pubmed:author | pubmed-author:TreumannAA | lld:pubmed |
| pubmed-article:7890742 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7890742 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:7890742 | pubmed:volume | 270 | lld:pubmed |
| pubmed-article:7890742 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7890742 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7890742 | pubmed:pagination | 6088-99 | lld:pubmed |
| pubmed-article:7890742 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:7890742 | pubmed:meshHeading | pubmed-meshheading:7890742-... | lld:pubmed |
| pubmed-article:7890742 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7890742 | pubmed:articleTitle | Primary structure of CD52. | lld:pubmed |
| pubmed-article:7890742 | pubmed:affiliation | Department of Biochemistry, University of Dundee, Scotland, United Kingdom. | lld:pubmed |
| pubmed-article:7890742 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7890742 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7890742 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| entrez-gene:1043 | entrezgene:pubmed | pubmed-article:7890742 | lld:entrezgene |
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