| pubmed-article:7890610 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7890610 | lifeskim:mentions | umls-concept:C0007452 | lld:lifeskim |
| pubmed-article:7890610 | lifeskim:mentions | umls-concept:C0003601 | lld:lifeskim |
| pubmed-article:7890610 | lifeskim:mentions | umls-concept:C0040663 | lld:lifeskim |
| pubmed-article:7890610 | lifeskim:mentions | umls-concept:C2355587 | lld:lifeskim |
| pubmed-article:7890610 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:7890610 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:7890610 | pubmed:dateCreated | 1995-4-14 | lld:pubmed |
| pubmed-article:7890610 | pubmed:abstractText | The interaction of the bovine opsin apoprotein with transducin in rod outer segment membranes was investigated using a guanyl nucleotide exchange assay. In exhaustive binding experiments, opsin activates transducin, with half-maximal exchange activity occurring at 0.8 mol of opsin/mol of transducin. The opsin activity was light-insensitive, hydroxylamine-resistant, unaffected by stoichiometric concentrations of retinaloxime, and more heat-labile than rhodopsin. The t1/2 of transducin activation in the presence of excess opsin was 8.5 min, compared with 0.7 min for metarhodopsin (II). The second-order rate constants were determined to be 0.012 pmol of guanosine 5'-(gamma-thio)triphosphate (GTP gamma S) bound per min/nM opsin and 0.35 pmol of GTP gamma S bound per min/nM metarhodopsin (II). Opsin was able to activate more than one transducin, although there appeared to be a turnover-dependent inactivation of the apoprotein. Opsin showed a broad pH range (5.8-7.4) for optimal activity, with no activity in buffers of pH > 9, whereas metarhodopsin (II) exhibited activity at pH > 9. Regulation of opsin activity by stoichiometric amounts of retinal was observed, with inhibition by 11-cis-retinal and stimulation by all-trans-retinal. A model for opsin activity is proposed. | lld:pubmed |
| pubmed-article:7890610 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7890610 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7890610 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7890610 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7890610 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:7890610 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:7890610 | pubmed:author | pubmed-author:FosterK WKW | lld:pubmed |
| pubmed-article:7890610 | pubmed:author | pubmed-author:KnoxB EBE | lld:pubmed |
| pubmed-article:7890610 | pubmed:author | pubmed-author:SuryaAA | lld:pubmed |
| pubmed-article:7890610 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7890610 | pubmed:day | 10 | lld:pubmed |
| pubmed-article:7890610 | pubmed:volume | 270 | lld:pubmed |
| pubmed-article:7890610 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7890610 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7890610 | pubmed:pagination | 5024-31 | lld:pubmed |
| pubmed-article:7890610 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:meshHeading | pubmed-meshheading:7890610-... | lld:pubmed |
| pubmed-article:7890610 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7890610 | pubmed:articleTitle | Transducin activation by the bovine opsin apoprotein. | lld:pubmed |
| pubmed-article:7890610 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, State University of New York Health Science Center, Syracuse 13210. | lld:pubmed |
| pubmed-article:7890610 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7890610 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7890610 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7890610 | lld:pubmed |
