Linked Life Data

7870102

Source:http://linkedlifedata.com/resource/pubmed/id/7870102

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:7870102rdf:typepubmed:Citationlld:pubmed
pubmed-article:7870102lifeskim:mentionsumls-concept:C0027923lld:lifeskim
pubmed-article:7870102lifeskim:mentionsumls-concept:C0205147lld:lifeskim
pubmed-article:7870102lifeskim:mentionsumls-concept:C0596698lld:lifeskim
pubmed-article:7870102lifeskim:mentionsumls-concept:C0026882lld:lifeskim
pubmed-article:7870102lifeskim:mentionsumls-concept:C0040975lld:lifeskim
pubmed-article:7870102lifeskim:mentionsumls-concept:C0205263lld:lifeskim
pubmed-article:7870102pubmed:issue1-2lld:pubmed
pubmed-article:7870102pubmed:dateCreated1995-3-30lld:pubmed
pubmed-article:7870102pubmed:abstractTextThe mutagenicity of the trifunctional alkylating (or cross-linking) agent TEM (triethylenemelamine or 2,4,6-tris(1-aziridinyl)-1,3,5-triazine) in the adenine-3 (ad-3) region was studied with a two-component heterokaryon (H-12) of Neurospora crassa. The objective was to characterize the genetic damage produced by this chemical to determine the spectrum of specific-locus mutations induced in a lower eukaryotic organism and to compare this spectrum with that induced in the mouse. Specific-locus mutations in the ad-3 region of strain H-12 result from gene/point mutations, multiple-locus mutations, and multilocus deletion mutations at the closely linked ad-3A and ad-3B loci. These loci control two sequential biochemical reactions in the purine biosynthetic pathway. A 0.1 M solution of TEM was used to treat conidial suspensions of H-12 for 20, 40, 80, 120, or 170 min to obtain dose-response curves for (1) inactivation of conidia, and (2) the induction of specific-locus mutations in the ad-3 region. These experiments demonstrated that TEM is a strong mutagen (maximum forward-mutation frequency between 100 and 1000 ad-3 mutations per 10(6) survivors) for the induction of specific-locus mutations in the ad-3 region. Both biochemical and classical genetic tests were used to characterize the TEM-induced ad-3 mutations from each of the five treatment groups to distinguish between the different genotypic classes and subclasses. The overall data base from these genetic studies demonstrates that TEM-induced ad-3 mutations result predominantly (95.5% [769/805]) from gene/point mutations at the ad-3A and ad-3B loci, and from a low percentage (4.5% [36/805) of multilocus deletion mutations. In addition, TEM induces an unusually high frequency of multiple-locus mutations with sites of recessive lethal damage closely linked with the ad-3 region. Comparison of the dose-response curves for the major classes and subclasses of TEM-induced ad-3 mutations demonstrates (1) that gene/point mutations and multilocus deletion mutations increase as the 1.4 power of TEM treatment time, and (2) that the two classes of TEM-induced multiple-locus ad-3 mutations consisting of gene/point mutations with separate sites of recessive lethal damage increase at about the 1.96 power of TEM treatment time. When the data from the present specific-locus studies are compared with those in the mouse, we find, insofar as such comparisons are possible, that a similar spectrum of specific-locus mutations has been induced by TEM in each assay system.lld:pubmed
pubmed-article:7870102pubmed:languageenglld:pubmed
pubmed-article:7870102pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7870102pubmed:citationSubsetIMlld:pubmed
pubmed-article:7870102pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7870102pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7870102pubmed:statusMEDLINElld:pubmed
pubmed-article:7870102pubmed:monthMarlld:pubmed
pubmed-article:7870102pubmed:issn0027-5107lld:pubmed
pubmed-article:7870102pubmed:authorpubmed-author:MallingH VHVlld:pubmed
pubmed-article:7870102pubmed:authorpubmed-author:de SerresF...lld:pubmed
pubmed-article:7870102pubmed:issnTypePrintlld:pubmed
pubmed-article:7870102pubmed:volume327lld:pubmed
pubmed-article:7870102pubmed:geneSymbolad-3lld:pubmed
pubmed-article:7870102pubmed:ownerNLMlld:pubmed
pubmed-article:7870102pubmed:authorsCompleteYlld:pubmed
pubmed-article:7870102pubmed:pagination87-111lld:pubmed
pubmed-article:7870102pubmed:dateRevised2006-11-15lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:meshHeadingpubmed-meshheading:7870102-...lld:pubmed
pubmed-article:7870102pubmed:year1995lld:pubmed
pubmed-article:7870102pubmed:articleTitleTriethylenemelamine: induction of specific-locus mutations in the ad-3 region of heterokaryon 12 of Neurospora crassa.lld:pubmed
pubmed-article:7870102pubmed:affiliationToxicology Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709-2233.lld:pubmed
pubmed-article:7870102pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7870102pubmed:publicationTypeComparative Studylld:pubmed