| pubmed-article:7849571 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7849571 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:7849571 | lifeskim:mentions | umls-concept:C0033692 | lld:lifeskim |
| pubmed-article:7849571 | lifeskim:mentions | umls-concept:C0033689 | lld:lifeskim |
| pubmed-article:7849571 | lifeskim:mentions | umls-concept:C2326566 | lld:lifeskim |
| pubmed-article:7849571 | lifeskim:mentions | umls-concept:C0205470 | lld:lifeskim |
| pubmed-article:7849571 | lifeskim:mentions | umls-concept:C1441547 | lld:lifeskim |
| pubmed-article:7849571 | lifeskim:mentions | umls-concept:C0486805 | lld:lifeskim |
| pubmed-article:7849571 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:7849571 | pubmed:dateCreated | 1995-3-14 | lld:pubmed |
| pubmed-article:7849571 | pubmed:abstractText | Neurocan is a brain-unique chondroitin sulfate proteoglycan (CSPG) whose expression and proteolytic cleavage are developmentally regulated. One of the proteolytic products (C-terminal half) is known to be a CSPG with a 150 kDa core glycoprotein (CSPG-150). To identify the N-terminal half of neurocan, we raised an anti-neurocan polyclonal antibody (PAb 291) using a synthetic peptide whose amino acid sequence matched a part of the N-terminal half of neurocan. Western blots showed that PAb 291 recognized two CSPGs, one with a 220 kDa core glycoprotein (CSPG-220, namely neurocan) and one with a 130 kDa core glycoprotein (CSPG-130) isolated from young rat brains. CSPG-130 was co-purified along with CSPG-220 by PAb 291-immunoaffinity column chromatography. The amino acid sequence of the N-terminus of the immunopurified CSPG-130 was exactly the same as the N-terminal sequence of CSPG-220. These results suggest that not only the C-terminal half (CSPG-150) but also the N-terminal half (CSPG-130) of CSPG-220 exists in a CSPG form in rat brain. Using PAb 291 and monoclonal antibody 1G2 (MAb 1G2) which recognizes CSPG-150 in addition to CSPG-220, we found that the contents of CSPG-130 and CSPG-150 in the rat brain reached maximum levels around the time of birth. Both CSPG-130 and 150 were observed, while CSPG-220 was hardly detectable in extracts from the adult rat brain. Immunohistochemical investigation showed that the PAb 291 antigen had a similar distribution pattern to the MAb 1G2 antigen.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:7849571 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7849571 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7849571 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7849571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7849571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7849571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7849571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7849571 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7849571 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7849571 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7849571 | pubmed:issn | 0197-0186 | lld:pubmed |
| pubmed-article:7849571 | pubmed:author | pubmed-author:WatanabeEE | lld:pubmed |
| pubmed-article:7849571 | pubmed:author | pubmed-author:OohiraAA | lld:pubmed |
| pubmed-article:7849571 | pubmed:author | pubmed-author:MatsuiFF | lld:pubmed |
| pubmed-article:7849571 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7849571 | pubmed:volume | 25 | lld:pubmed |
| pubmed-article:7849571 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7849571 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7849571 | pubmed:pagination | 425-31 | lld:pubmed |
| pubmed-article:7849571 | pubmed:dateRevised | 2010-11-18 | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:meshHeading | pubmed-meshheading:7849571-... | lld:pubmed |
| pubmed-article:7849571 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7849571 | pubmed:articleTitle | Immunological identification of two proteoglycan fragments derived from neurocan, a brain-specific chondroitin sulfate proteoglycan. | lld:pubmed |
| pubmed-article:7849571 | pubmed:affiliation | Department of Perinatology, Institute for Developmental Research, Aichi, Japan. | lld:pubmed |
| pubmed-article:7849571 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7849571 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7849571 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7849571 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7849571 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7849571 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7849571 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7849571 | lld:pubmed |
