| pubmed-article:7836927 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7836927 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:7836927 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:7836927 | lifeskim:mentions | umls-concept:C0061877 | lld:lifeskim |
| pubmed-article:7836927 | lifeskim:mentions | umls-concept:C1621967 | lld:lifeskim |
| pubmed-article:7836927 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7836927 | lifeskim:mentions | umls-concept:C1518071 | lld:lifeskim |
| pubmed-article:7836927 | pubmed:issue | 2 | lld:pubmed |
| pubmed-article:7836927 | pubmed:dateCreated | 1995-2-24 | lld:pubmed |
| pubmed-article:7836927 | pubmed:abstractText | During development, thymocytes express a number of genes typical for activated peripheral T lymphocytes, including granzymes. We have now analyzed by reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry, and cytochemistry fetal liver cells and thymocytes at various developmental stages for the expression of granzyme A-G genes. At days 13-17 of gestation, only granzyme B but none of the other granzymes is expressed in fetal liver. In the most immature, Pgp-1+IL2R alpha-, thymocyte subpopulation mRNAs for granzymes A-C but not for granzymes D-G are detectable. Upon further differentiation via Pgp-1-IL-2R alpha + into more mature Pgp-1-IL-2R alpha- thymocytes the level of expression of granzymes A, B, and C gradually declines reaching its lowest level at the CD4+ 8+ double positive stage. In fetal thymic lobes depleted of lymphoid cells by treatment with deoxyguanosine, no transcripts for granzymes A, B, and C were found indicating that the PCR signals are derived exclusively from early precursor T/natural killer (NK) lineage cells rather than from residual stromal elements. In mature CD4+CD8- and CD4-CD8+ thymocytes, granzyme B mRNA is found at similar levels in both subsets whereas granzyme A mRNA is expressed selectively in the CD4-CD8+ subset. Enzymatic activity of granzyme A was only seen in a fraction of CD4-CD8+ thymocytes negative for heat stable antigen (HSA) but not in the more immature HSA+ fraction of CD4-CD8+ thymocytes. The data suggest that (a) granzyme B is a pro-thymocyte marker for all T/NK lineage cells; (b) granzyme A transcripts are associated with thymocytes with the potential to develop into the CD8+ lineage; and (c) granzyme A enzymatic activity is only expressed in the most mature CD4-CD8+ stage, suggesting that granzyme proteins are not involved in early stages of thymocyte development. | lld:pubmed |
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| pubmed-article:7836927 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7836927 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7836927 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7836927 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7836927 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:7836927 | pubmed:issn | 0022-1007 | lld:pubmed |
| pubmed-article:7836927 | pubmed:author | pubmed-author:EichmannKK | lld:pubmed |
| pubmed-article:7836927 | pubmed:author | pubmed-author:SimonM MMM | lld:pubmed |
| pubmed-article:7836927 | pubmed:author | pubmed-author:TranT TTT | lld:pubmed |
| pubmed-article:7836927 | pubmed:author | pubmed-author:LeveltC NCN | lld:pubmed |
| pubmed-article:7836927 | pubmed:author | pubmed-author:EbnetKK | lld:pubmed |
| pubmed-article:7836927 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7836927 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:7836927 | pubmed:volume | 181 | lld:pubmed |
| pubmed-article:7836927 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7836927 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7836927 | pubmed:pagination | 755-63 | lld:pubmed |
| pubmed-article:7836927 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7836927 | pubmed:meshHeading | pubmed-meshheading:7836927-... | lld:pubmed |
| pubmed-article:7836927 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7836927 | pubmed:articleTitle | Transcription of granzyme A and B genes is differentially regulated during lymphoid ontogeny. | lld:pubmed |
| pubmed-article:7836927 | pubmed:affiliation | Max-Planck-Institute for Immunobiology, Freiburg, Germany. | lld:pubmed |
| pubmed-article:7836927 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7836927 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7836927 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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