Linked Life Data

7833803

Source:http://linkedlifedata.com/resource/pubmed/id/7833803

Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:7833803rdf:typepubmed:Citationlld:pubmed
pubmed-article:7833803lifeskim:mentionsumls-concept:C1705165lld:lifeskim
pubmed-article:7833803lifeskim:mentionsumls-concept:C0022702lld:lifeskim
pubmed-article:7833803lifeskim:mentionsumls-concept:C1710706lld:lifeskim
pubmed-article:7833803lifeskim:mentionsumls-concept:C0185026lld:lifeskim
pubmed-article:7833803lifeskim:mentionsumls-concept:C0936012lld:lifeskim
pubmed-article:7833803lifeskim:mentionsumls-concept:C0392762lld:lifeskim
pubmed-article:7833803lifeskim:mentionsumls-concept:C2700061lld:lifeskim
pubmed-article:7833803lifeskim:mentionsumls-concept:C0052987lld:lifeskim
pubmed-article:7833803pubmed:issue9lld:pubmed
pubmed-article:7833803pubmed:dateCreated1995-3-1lld:pubmed
pubmed-article:7833803pubmed:abstractTextThe fluorescence-monitored kinetics of folding and unfolding of barstar by guanidine hydrochloride (GdnHCl) in the folding transition zone, at pH 7, 25 degrees C, have been quantitatively analyzed using a 3-state mechanism: U(S)<-->UF<-->N. U(S) and UF are slow-refolding and fast-refolding unfolded forms of barstar, and N is the native protein. U(S) and UF probably differ in possessing trans and cis conformations, respectively, of the Tyr 47-Pro 48 bond. The 3-state model could be used because the kinetics of folding and unfolding of barstar show 2 phases, a fast phase and a slow phase, and because the relative amplitudes of the 2 phases depend only on the final refolding conditions and not on the initial conditions. Analysis of the observed kinetics according to the 3-state model yields the values of the 4 microscopic rate constants that describe the transitions between the 3 states at different concentrations of GdnHCl. The value of the equilibrium unfolded ratio U(S):UF (K21) and the values of the rate constants of the U(S)-->UF and UF-->U(S) reactions, k12 and k21, respectively, are shown to be independent of the concentration of GdnHCl. K21 has a value of 2.1 +/- 0.1, and k12 and k21 have values of 5.3 x 10(-3) s-1 and 11.2 x 10(-3) s-1, respectively. Double-jump experiments that monitor reactions that are silent to fluorescence monitoring were used to confirm the values of K21, k12, and k21 obtained from the 3-state analysis and thereby the validity of the 3-state model.(ABSTRACT TRUNCATED AT 250 WORDS)lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:languageenglld:pubmed
pubmed-article:7833803pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:citationSubsetIMlld:pubmed
pubmed-article:7833803pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7833803pubmed:statusMEDLINElld:pubmed
pubmed-article:7833803pubmed:monthSeplld:pubmed
pubmed-article:7833803pubmed:issn0961-8368lld:pubmed
pubmed-article:7833803pubmed:authorpubmed-author:UdgaonkarJ...lld:pubmed
pubmed-article:7833803pubmed:authorpubmed-author:ShastryM CMClld:pubmed
pubmed-article:7833803pubmed:authorpubmed-author:AgasheV RVRlld:pubmed
pubmed-article:7833803pubmed:issnTypePrintlld:pubmed
pubmed-article:7833803pubmed:volume3lld:pubmed
pubmed-article:7833803pubmed:ownerNLMlld:pubmed
pubmed-article:7833803pubmed:authorsCompleteYlld:pubmed
pubmed-article:7833803pubmed:pagination1409-17lld:pubmed
pubmed-article:7833803pubmed:dateRevised2009-11-18lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:meshHeadingpubmed-meshheading:7833803-...lld:pubmed
pubmed-article:7833803pubmed:year1994lld:pubmed
pubmed-article:7833803pubmed:articleTitleQuantitative analysis of the kinetics of denaturation and renaturation of barstar in the folding transition zone.lld:pubmed
pubmed-article:7833803pubmed:affiliationNational Centre For Biological Sciences, TIFR Centre, Indian Institute of Science Campus, Bangalore.lld:pubmed
pubmed-article:7833803pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7833803pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:7833803lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:7833803lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:7833803lld:pubmed
http://linkedlifedata.com/r...pubmed:referesTopubmed-article:7833803lld:pubmed