| pubmed-article:7828549 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7828549 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:7828549 | lifeskim:mentions | umls-concept:C0029418 | lld:lifeskim |
| pubmed-article:7828549 | lifeskim:mentions | umls-concept:C0012472 | lld:lifeskim |
| pubmed-article:7828549 | lifeskim:mentions | umls-concept:C0021665 | lld:lifeskim |
| pubmed-article:7828549 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:7828549 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7828549 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7828549 | pubmed:dateCreated | 1995-2-21 | lld:pubmed |
| pubmed-article:7828549 | pubmed:abstractText | Insulin-like growth factor I (IGF-I) is a widely expressed abundant autocrine and paracrine factor that regulates the proliferation and differentiation of a variety of cell types. Prostaglandin E2 (PGE2) is a potent stimulator of IGF-I synthesis in bone. We examined the regulation of IGF-I synthesis by PGE2 in osteoblast-enriched (Ob) cells from fetal rat calvaria. PGE2 treatment of Ob cells at 1 microM for 2 h resulted in a 5-fold increase in heterogeneous nuclear RNA levels, as measured by a reverse transcriptase-polymerase chain reaction assay, suggesting an increase in IGF-I gene transcription. RNase protection analysis was used to map the transcriptional start sites in the IGF-I gene that are used in Ob cells. Consistent with other extrahepatic tissues, initiation of transcription occurs primarily at three sites within the 5'-regions of exon 1 of the IGF-I gene. PGE2 treatment did not alter start site usage. The regions upstream of these transcriptional start sites were analyzed by transiently transfecting Ob cells with putative rat IGF-I promoter sequences ligated to a luciferase reporter gene. Constructs containing 1.4 kilobases of the 5'-regions regions of exons 1 and 2 had significant promoter activity. PGE2 treatment of transfected Ob cells increased luciferase activity 5-fold when a 1.4-kilobase exon 1 promoter fragment was tested. This increase in luciferase activity was time and dose dependent. Smaller regions of the exon 1 promoter sequence gave higher basal activity and were less responsive to PGE2. We conclude that regions involved in IGF-I regulation by PGE2 are contained within the IGF-I promoter. | lld:pubmed |
| pubmed-article:7828549 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7828549 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:7828549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7828549 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7828549 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:7828549 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:7828549 | pubmed:author | pubmed-author:CanalisEE | lld:pubmed |
| pubmed-article:7828549 | pubmed:author | pubmed-author:LeRoithDD | lld:pubmed |
| pubmed-article:7828549 | pubmed:author | pubmed-author:RobertsC... | lld:pubmed |
| pubmed-article:7828549 | pubmed:author | pubmed-author:AdamsM PMP | lld:pubmed |
| pubmed-article:7828549 | pubmed:author | pubmed-author:DelanyA MAM | lld:pubmed |
| pubmed-article:7828549 | pubmed:author | pubmed-author:PashJ MJM | lld:pubmed |
| pubmed-article:7828549 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7828549 | pubmed:volume | 136 | lld:pubmed |
| pubmed-article:7828549 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7828549 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7828549 | pubmed:pagination | 33-8 | lld:pubmed |
| pubmed-article:7828549 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
| pubmed-article:7828549 | pubmed:meshHeading | pubmed-meshheading:7828549-... | lld:pubmed |
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| pubmed-article:7828549 | pubmed:meshHeading | pubmed-meshheading:7828549-... | lld:pubmed |
| pubmed-article:7828549 | pubmed:meshHeading | pubmed-meshheading:7828549-... | lld:pubmed |
| pubmed-article:7828549 | pubmed:meshHeading | pubmed-meshheading:7828549-... | lld:pubmed |
| pubmed-article:7828549 | pubmed:meshHeading | pubmed-meshheading:7828549-... | lld:pubmed |
| pubmed-article:7828549 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7828549 | pubmed:articleTitle | Regulation of insulin-like growth factor I transcription by prostaglandin E2 in osteoblast cells. | lld:pubmed |
| pubmed-article:7828549 | pubmed:affiliation | Department of Research, Saint Francis Hospital and Medical Center, Hartford, Connecticut 06105. | lld:pubmed |
| pubmed-article:7828549 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7828549 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7828549 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7828549 | lld:pubmed |
