| pubmed-article:7821961 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7821961 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7821961 | lifeskim:mentions | umls-concept:C0040421 | lld:lifeskim |
| pubmed-article:7821961 | lifeskim:mentions | umls-concept:C0836921 | lld:lifeskim |
| pubmed-article:7821961 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:7821961 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:7821961 | lifeskim:mentions | umls-concept:C0521115 | lld:lifeskim |
| pubmed-article:7821961 | lifeskim:mentions | umls-concept:C1515655 | lld:lifeskim |
| pubmed-article:7821961 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7821961 | pubmed:dateCreated | 1995-2-16 | lld:pubmed |
| pubmed-article:7821961 | pubmed:abstractText | Accumulating data indicate that cytokines, peptides involved in regulation of both physiological and pathological immune responses, are produced predominantly at the site of local antigen stimulation. Cytokine-producing cells were detected at the protein level in human tonsil tissue obtained from children with recurrent tonsillitis or infectious mononucleosis (IM). Concomitant production of 19 different human cytokines, interleukin-1 alpha (IL-1 alpha), IL-1 beta, IL-1 receptor antagonist (ra), IL-2, IL-3, IL-4, IL-5, IL-6, IL-8, IL-10, IL-13, granulocyte-macrophage colony-stimulating factor (GM-CSF), G-CSF, tumour necrosis factor-alpha (TNF-alpha), TNF-beta, interferon-gamma (IFN-gamma) and transforming growth factor-beta 1-3 (TGF-beta 1-3), was identified at a single-cell level by indirect immunohistochemical staining procedures and use of carefully selected cytokine-specific antibodies (Ab). Fresh frozen sections were fixed with 4% paraformaldehyde and permeabilized by 0.1% saponin treatment, eluting cholesterol from the cell-surface membrane and the Golgi complex. The intracellular localization of all cytokines, except IL-1 and IL-1ra, was demonstrated by a characteristic local cytoplasmic perinuclear configuration in producer cells. In addition, the immunoreactivity for certain cytokines (IL-2, IL-4, IL-5, G-CSF and GM-CSF) was expressed on the cell membranes and extended over a large extracellular area encompassing the producer cell. Localization of the cytokine to the Golgi organelle was established by co-staining with a monoclonal antibody (mAb) specific to the Golgi complex. Both the extra- and intracellular cytokine staining reactions could be blocked by preincubation of the cytokine-specific Ab with the corresponding purified natural or recombinant cytokine. A complex cytokine pattern was established in both groups studied, where most T-helper type 1 (Th1) and Th2 lymphokines were expressed in the tonsils but at different frequencies and localizations. Cells expressing IL-4, IL-5, IL-10 and IL-13, (Th2 response) were evident at higher frequencies in recurrent tonsillitis compared to sections from IM, which were associated with a more pronounced IL-2, IFN-gamma and TNF-beta expression. | lld:pubmed |
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| pubmed-article:7821961 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7821961 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7821961 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7821961 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7821961 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:7821961 | pubmed:issn | 0019-2805 | lld:pubmed |
| pubmed-article:7821961 | pubmed:author | pubmed-author:AnderssonJJ | lld:pubmed |
| pubmed-article:7821961 | pubmed:author | pubmed-author:AbramsJJ | lld:pubmed |
| pubmed-article:7821961 | pubmed:author | pubmed-author:BjörkLL | lld:pubmed |
| pubmed-article:7821961 | pubmed:author | pubmed-author:DoraG LGL | lld:pubmed |
| pubmed-article:7821961 | pubmed:author | pubmed-author:AnderssonUU | lld:pubmed |
| pubmed-article:7821961 | pubmed:author | pubmed-author:AgrenKK | lld:pubmed |
| pubmed-article:7821961 | pubmed:author | pubmed-author:LittonMM | lld:pubmed |
| pubmed-article:7821961 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7821961 | pubmed:volume | 83 | lld:pubmed |
| pubmed-article:7821961 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7821961 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7821961 | pubmed:pagination | 16-24 | lld:pubmed |
| pubmed-article:7821961 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7821961 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7821961 | pubmed:articleTitle | Concomitant in vivo production of 19 different cytokines in human tonsils. | lld:pubmed |
| pubmed-article:7821961 | pubmed:affiliation | Department of Immunology, Arrhenius Laboratories for Natural Sciences, Stockholm University, Sweden. | lld:pubmed |
| pubmed-article:7821961 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7821961 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7821961 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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