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pubmed-article:7812800pubmed:abstractTextRats received bilateral intracerebroventricular (ICV) infusions of either AF64A (1.5 nmol/ventricle; n = 9) or vehicle (3.0 microliters/ventricle; n = 7). Four weeks later, the animals were anesthetized and their brains processed to visualize and quantify choline acetyltransferase (ChAT) immunoreactive (IR) and parvalbumin-IR GABAergic neurons in the septal complex by immunocytochemistry (PAP method). AF64A significantly reduced the number of ChAT-IR perikarya in the medial septum (28%), ventral limb of the diagonal band of Broca (30%), and horizontal limb of the diagonal band of Broca (20%), but did not affect the number of parvalbumin-containing GABAergic neurons in any of the septal subdivisions. These results provide further evidence that AF64A is a selective cholinotoxin.lld:pubmed
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pubmed-article:7812800pubmed:articleTitleAF64A affects septal choline acetyltransferase but not parvalbumin immunoreactive cells.lld:pubmed
pubmed-article:7812800pubmed:affiliationDepartment of Pharmacology and Experimental Therapeutics, Loyola University Chicago, Stritch School of Medicine, Maywood, IL 60153.lld:pubmed
pubmed-article:7812800pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7812800pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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