| pubmed-article:7810685 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C1267092 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C0034052 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C0230445 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C0205145 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C0038836 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C1511790 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C0033268 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C0442726 | lld:lifeskim |
| pubmed-article:7810685 | lifeskim:mentions | umls-concept:C0126266 | lld:lifeskim |
| pubmed-article:7810685 | pubmed:issue | 6 Pt 1 | lld:pubmed |
| pubmed-article:7810685 | pubmed:dateCreated | 1995-1-31 | lld:pubmed |
| pubmed-article:7810685 | pubmed:abstractText | Sources of superoxide anion (O2-.) production in calf pulmonary artery smooth muscle homogenate and subcellular fractions were examined in this study by measurement of the chemiluminescence produced by the reaction of O2-. with 50 microM lucigenin, because recent evidence suggests that endogenously produced reactive O2 species appear to mediate certain vascular responses. In the homogenate fraction, an NADH (0.1 mM)-dependent oxidoreductase activity was the major detected source of chemiluminescence. NADPH (0.1 mM) produced only 3% of the O2-. observed with NADH. Quantitation of certain other potential sources of O2-. (under optimized conditions), including xanthine oxidase (0.1 mM hypoxanthine), mitochondria (5 mM succinate + 30 microM antimycin), cyclooxygenase/lipoxygenase (1 microM arachidonic acid + 0.1 mM NADPH), or autooxidation (0.1 mg/ml superoxide dismutase), resulted in the detection of minimal amounts (< 3% of NADH) of chemiluminescence. Estimation of mitochondrial O2-. production from tissue respiration rates suggests that lucigenin is a poor detector of intramitochondrial O2-.. These observations were confirmed by examination of chemiluminescence produced by subcellular fractions, where the major activity detected was an NADH oxidoreductase, which fractionated in a manner closely matching the activity of the microsomal marker enzyme rotenone-insensitive NADH-cytochrome c reductase. Because this NADH oxidoreductase appears to be a major vascular smooth muscle-derived source of O2-. production, this system has the potential to be an important endogenous source for the generation of vasoactive reactive O2 species. | lld:pubmed |
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| pubmed-article:7810685 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7810685 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7810685 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7810685 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7810685 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7810685 | pubmed:issn | 0002-9513 | lld:pubmed |
| pubmed-article:7810685 | pubmed:author | pubmed-author:WolinM SMS | lld:pubmed |
| pubmed-article:7810685 | pubmed:author | pubmed-author:MohazzabK MKM | lld:pubmed |
| pubmed-article:7810685 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7810685 | pubmed:volume | 267 | lld:pubmed |
| pubmed-article:7810685 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7810685 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7810685 | pubmed:pagination | L815-22 | lld:pubmed |
| pubmed-article:7810685 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:7810685 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7810685 | pubmed:articleTitle | Sites of superoxide anion production detected by lucigenin in calf pulmonary artery smooth muscle. | lld:pubmed |
| pubmed-article:7810685 | pubmed:affiliation | Department of Physiology, New York Medical College, Valhalla 10595. | lld:pubmed |
| pubmed-article:7810685 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7810685 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7810685 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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