| pubmed-article:7809117 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7809117 | lifeskim:mentions | umls-concept:C0162740 | lld:lifeskim |
| pubmed-article:7809117 | lifeskim:mentions | umls-concept:C1721101 | lld:lifeskim |
| pubmed-article:7809117 | lifeskim:mentions | umls-concept:C0206524 | lld:lifeskim |
| pubmed-article:7809117 | lifeskim:mentions | umls-concept:C1274040 | lld:lifeskim |
| pubmed-article:7809117 | lifeskim:mentions | umls-concept:C0441889 | lld:lifeskim |
| pubmed-article:7809117 | lifeskim:mentions | umls-concept:C0032521 | lld:lifeskim |
| pubmed-article:7809117 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:7809117 | pubmed:issue | 26 | lld:pubmed |
| pubmed-article:7809117 | pubmed:dateCreated | 1995-2-2 | lld:pubmed |
| pubmed-article:7809117 | pubmed:abstractText | In the bacterium Alcaligenes eutrophus, three genes encode the enzymes necessary to catalyze the synthesis of poly[(R)-(-)-3-hydroxybutyrate] (PHB) from acetyl-CoA. In order to target these enzymes into the plastids of higher plants, the genes were modified by addition of DNA fragments encoding a pea chloroplast transit peptide, a constitutive plant promoter, and a poly(A) addition sequence. Each of the modified bacterial genes was introduced into Arabidopsis thaliana by Agrobacterium-mediated transformation, and plants containing all three genes were obtained by sexual crosses. These plants accumulated PHB up to 14% of the dry weight as 0.2- to 0.7-micron granules within plastids. In contrast to earlier experiments in which expression of the PHB biosynthetic pathway in the cytoplasm led to a deleterious effect on growth, expression of the PHB biosynthetic pathway in plastids had no obvious effect on the growth or fertility of the transgenic plants and resulted in a 100-fold increase in the amount of PHB that accumulated. We conclude that there does not appear to be any biological barrier to high-level production of PHB in higher plants. The high level of PHB accumulation also suggests that the synthesis of plastid acetyl-CoA is regulated by a mechanism which responds to metabolic demand. | lld:pubmed |
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| pubmed-article:7809117 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7809117 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7809117 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7809117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7809117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7809117 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7809117 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7809117 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7809117 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:7809117 | pubmed:author | pubmed-author:SomervilleCC | lld:pubmed |
| pubmed-article:7809117 | pubmed:author | pubmed-author:PoirierYY | lld:pubmed |
| pubmed-article:7809117 | pubmed:author | pubmed-author:NawrathCC | lld:pubmed |
| pubmed-article:7809117 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7809117 | pubmed:day | 20 | lld:pubmed |
| pubmed-article:7809117 | pubmed:volume | 91 | lld:pubmed |
| pubmed-article:7809117 | pubmed:geneSymbol | phbA | lld:pubmed |
| pubmed-article:7809117 | pubmed:geneSymbol | phbC | lld:pubmed |
| pubmed-article:7809117 | pubmed:geneSymbol | phbB | lld:pubmed |
| pubmed-article:7809117 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7809117 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7809117 | pubmed:pagination | 12760-4 | lld:pubmed |
| pubmed-article:7809117 | pubmed:dateRevised | 2010-9-13 | lld:pubmed |
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| pubmed-article:7809117 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7809117 | pubmed:articleTitle | Targeting of the polyhydroxybutyrate biosynthetic pathway to the plastids of Arabidopsis thaliana results in high levels of polymer accumulation. | lld:pubmed |
| pubmed-article:7809117 | pubmed:affiliation | Department of Plant Biology, Carnegie Institution of Washington, Stanford, CA 94305. | lld:pubmed |
| pubmed-article:7809117 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7809117 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
| pubmed-article:7809117 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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