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pubmed-article:7803131pubmed:abstractTextThe polymerase chain reaction (PCR)-based method for screening pooled yeast artificial chromosome (YAC) libraries was modified by adding a step for the restriction enzyme digestion of the PCR products. This modification significantly increased the reliability of YAC screening and made it possible to identify YAC clones without a cumbersome verification step by colony hybridization and/or Southern blotting. Using this method, we assigned 39 mouse YAC clones to a mouse genetic map with 13 biallelic, polymorphic expressed sequence tags. This method provides a fast, reliable way to identify YAC clones with PCR-based sequence-tagged sites.lld:pubmed
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pubmed-article:7803131pubmed:authorpubmed-author:ShawM WMWlld:pubmed
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pubmed-article:7803131pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:7803131pubmed:year1994lld:pubmed
pubmed-article:7803131pubmed:articleTitleSimple and robust screening of pooled yeast artificial chromosome libraries by the restriction enzyme digestion of polymerase chain reaction products.lld:pubmed
pubmed-article:7803131pubmed:affiliationCenter for Molecular Medicine and Genetics, Wayne State University School of Medicine, Detroit, Michigan.lld:pubmed
pubmed-article:7803131pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7803131pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
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