| pubmed-article:7789279 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7789279 | lifeskim:mentions | umls-concept:C0013574 | lld:lifeskim |
| pubmed-article:7789279 | lifeskim:mentions | umls-concept:C0007589 | lld:lifeskim |
| pubmed-article:7789279 | lifeskim:mentions | umls-concept:C1705920 | lld:lifeskim |
| pubmed-article:7789279 | lifeskim:mentions | umls-concept:C0036488 | lld:lifeskim |
| pubmed-article:7789279 | lifeskim:mentions | umls-concept:C1511938 | lld:lifeskim |
| pubmed-article:7789279 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:7789279 | pubmed:dateCreated | 1995-7-27 | lld:pubmed |
| pubmed-article:7789279 | pubmed:abstractText | During early embryogenesis, the highly regulative sea urchin embryo relies extensively on cell-cell interactions for cellular specification. Here, the role of cellular interactions in the temporal and spatial expression of markers for oral and aboral ectoderm in Strongylocentrotus purpuratus and Lytechinus pictus was investigated. When pairs of mesomeres or animal caps, which are fated to give rise to ectoderm, were isolated and cultured they developed into ciliated embryoids that were morphologically polarized. In animal explants from S. purpuratus, the aboral ectoderm-specific Spec1 gene was activated at the same time as in control embryos and at relatively high levels. The Spec1 protein was restricted to the squamous epithelial cells in the embryoids suggesting that an oral-aboral axis formed and aboral ectoderm differentiation occurred correctly. However, the Ecto V protein, a marker for oral ectoderm differentiation, was detected throughout the embryoid and no stomodeum or ciliary band formed. These results indicated that animal explants from S. purpuratus were autonomous in their ability to form an oral-aboral axis and to differentiate aboral ectoderm, but other aspects of ectoderm differentiation require interaction with vegetal blastomeres. In contrast to S. purpuratus, aboral ectoderm-specific genes were not expressed in animal explants from L. pictus even though the resulting embryoids were morphologically very similar to those of S. purpuratus. Recombination of the explants with vegetal blastomeres or exposure to the vegetalizing agent LiCl restored activity of aboral ectoderm-specific genes, suggesting the requirement of a vegetal induction for differentiation of aboral ectoderm cells.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:7789279 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7789279 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7789279 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7789279 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7789279 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7789279 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7789279 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7789279 | pubmed:month | May | lld:pubmed |
| pubmed-article:7789279 | pubmed:issn | 0950-1991 | lld:pubmed |
| pubmed-article:7789279 | pubmed:author | pubmed-author:BrandhorstB... | lld:pubmed |
| pubmed-article:7789279 | pubmed:author | pubmed-author:KleinW HWH | lld:pubmed |
| pubmed-article:7789279 | pubmed:author | pubmed-author:Wikramanayake... | lld:pubmed |
| pubmed-article:7789279 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7789279 | pubmed:volume | 121 | lld:pubmed |
| pubmed-article:7789279 | pubmed:geneSymbol | Spec1 | lld:pubmed |
| pubmed-article:7789279 | pubmed:geneSymbol | LpS1 | lld:pubmed |
| pubmed-article:7789279 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7789279 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7789279 | pubmed:pagination | 1497-505 | lld:pubmed |
| pubmed-article:7789279 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:meshHeading | pubmed-meshheading:7789279-... | lld:pubmed |
| pubmed-article:7789279 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7789279 | pubmed:articleTitle | Autonomous and non-autonomous differentiation of ectoderm in different sea urchin species. | lld:pubmed |
| pubmed-article:7789279 | pubmed:affiliation | Department of Biochemistry and Molecular Biology, University of Texas, Houston 77030, USA. | lld:pubmed |
| pubmed-article:7789279 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7789279 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7789279 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7789279 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7789279 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7789279 | lld:pubmed |
