Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:7775596rdf:typepubmed:Citationlld:pubmed
pubmed-article:7775596lifeskim:mentionsumls-concept:C0025914lld:lifeskim
pubmed-article:7775596lifeskim:mentionsumls-concept:C0026809lld:lifeskim
pubmed-article:7775596lifeskim:mentionsumls-concept:C0227525lld:lifeskim
pubmed-article:7775596lifeskim:mentionsumls-concept:C0376180lld:lifeskim
pubmed-article:7775596lifeskim:mentionsumls-concept:C0018270lld:lifeskim
pubmed-article:7775596lifeskim:mentionsumls-concept:C1948023lld:lifeskim
pubmed-article:7775596pubmed:issue3lld:pubmed
pubmed-article:7775596pubmed:dateCreated1995-7-11lld:pubmed
pubmed-article:7775596pubmed:abstractTextHepatocyte growth is regulated by various growth factors, including epidermal growth factor (EGF) and insulin. Recently, several additional peptide hormones have been shown to stimulate growth of hepatocyte only in the presence of EGF or insulin and are thus termed secondary mitogens. Gastrin regulates growth of normal and neoplastic gastrointestinal tissues, but the effect on growth of hepatocyte is unknown. We examined the effect of gastrin on growth of a normal mouse hepatocyte (NMH) line established in our laboratory. Effect of gastrin-17 (G-17) (10(-8) to 10(-6) M) on growth of NHM cells was examined in either the presence or absence of EGF in the culture medium. Growth of NMH cells was evaluated by incorporation of either bromodeoxyuridine (BrdU) or 3H-thymidine and by counting cells. Presence of a cell-surface receptor for G-17 was determined by Scatchard analysis using 125I-G-17. In the presence of EGF, gastrin stimulated growth of NMH cells; in the absence of EGF, gastrin did not affect growth. The stimulatory effect of gastrin on NMH cells was blocked by JMV 320, a CCK-B type receptor antagonist. NMH cells possess a single, high affinity binding site for gastrin (Kd = 1.2 nM); EGF increased the gastrin binding capacity compared to non-treated cells (3.5 +/- 0.4 vs. 2.2 +/- 0.6 fmol/10(6) cells). G-17 stimulated growth of NMH cells through a single high affinity receptor for G-17 which pharmcologically appears to be the CCK-B type only in the presence of EGF and thus can be considered a secondary mitogen.lld:pubmed
pubmed-article:7775596pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7775596pubmed:granthttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7775596pubmed:languageenglld:pubmed
pubmed-article:7775596pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7775596pubmed:citationSubsetIMlld:pubmed
pubmed-article:7775596pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7775596pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7775596pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:7775596pubmed:statusMEDLINElld:pubmed
pubmed-article:7775596pubmed:monthJunlld:pubmed
pubmed-article:7775596pubmed:issn0021-9541lld:pubmed
pubmed-article:7775596pubmed:authorpubmed-author:ThompsonJ CJClld:pubmed
pubmed-article:7775596pubmed:authorpubmed-author:TownsendC...lld:pubmed
pubmed-article:7775596pubmed:authorpubmed-author:IshizukaJJlld:pubmed
pubmed-article:7775596pubmed:authorpubmed-author:YanC YCYlld:pubmed
pubmed-article:7775596pubmed:authorpubmed-author:BoldR JRJlld:pubmed
pubmed-article:7775596pubmed:issnTypePrintlld:pubmed
pubmed-article:7775596pubmed:volume163lld:pubmed
pubmed-article:7775596pubmed:ownerNLMlld:pubmed
pubmed-article:7775596pubmed:authorsCompleteYlld:pubmed
pubmed-article:7775596pubmed:pagination532-7lld:pubmed
pubmed-article:7775596pubmed:dateRevised2007-11-14lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:meshHeadingpubmed-meshheading:7775596-...lld:pubmed
pubmed-article:7775596pubmed:year1995lld:pubmed
pubmed-article:7775596pubmed:articleTitleGrowth of mouse hepatocytes is stimulated by gastrin.lld:pubmed
pubmed-article:7775596pubmed:affiliationDepartment of Surgery, University of Texas Medical Branch, Galveston 77555, USA.lld:pubmed
pubmed-article:7775596pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7775596pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:7775596pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed