| pubmed-article:7707883 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7707883 | lifeskim:mentions | umls-concept:C0330390 | lld:lifeskim |
| pubmed-article:7707883 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:7707883 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:7707883 | lifeskim:mentions | umls-concept:C0597357 | lld:lifeskim |
| pubmed-article:7707883 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:7707883 | lifeskim:mentions | umls-concept:C0016904 | lld:lifeskim |
| pubmed-article:7707883 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:7707883 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:7707883 | pubmed:dateCreated | 1995-5-10 | lld:pubmed |
| pubmed-article:7707883 | pubmed:abstractText | Polymerase chain reaction was used to amplify the cDNA region that codes for the large intracellular loop of the beta 3 subunit of the gamma-aminobutyric acidA/benzodiazepine receptors (GABAAR/BZDR) from rat brain. The amplified cDNA was inserted into the prokaryotic expression vector pGEX-3X and a fusion protein containing glutathione-S-transferase and beta 3 intracellular loop moieties was expressed in bacteria. The fusion protein was affinity-purified and it was used to raise a rabbit anti-beta 3 antiserum. The anti-beta 3 antiserum immunoprecipitated the gamma-aminobutyric acidA receptor from rat and bovine brain. Immunoblots of the affinity-purified GABAAR/BZDR from bovine brain revealed that the anti-beta 3 antiserum reacted with a 57 kDa peptide, whereas the monoclonal antibody 62-3G1 that recognized both beta 2 and beta 3 reacted with 55 and 57 kDa peptides. The anti-beta 3 antiserum showed specificity for the beta 3 subunit vs beta 2 and beta 1. | lld:pubmed |
| pubmed-article:7707883 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7707883 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7707883 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7707883 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7707883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7707883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7707883 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7707883 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7707883 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:7707883 | pubmed:issn | 0169-328X | lld:pubmed |
| pubmed-article:7707883 | pubmed:author | pubmed-author:FernandoL PLP | lld:pubmed |
| pubmed-article:7707883 | pubmed:author | pubmed-author:KhanZ UZU | lld:pubmed |
| pubmed-article:7707883 | pubmed:author | pubmed-author:De BlasA LAL | lld:pubmed |
| pubmed-article:7707883 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7707883 | pubmed:volume | 28 | lld:pubmed |
| pubmed-article:7707883 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7707883 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7707883 | pubmed:pagination | 94-100 | lld:pubmed |
| pubmed-article:7707883 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:meshHeading | pubmed-meshheading:7707883-... | lld:pubmed |
| pubmed-article:7707883 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7707883 | pubmed:articleTitle | Antibodies to the rat beta 3 subunit of the gamma-aminobutyric acid A receptors. | lld:pubmed |
| pubmed-article:7707883 | pubmed:affiliation | Division of Molecular Biology and Biochemistry, School of Biological Sciences, University of Missouri-Kansas City 64110-2499. | lld:pubmed |
| pubmed-article:7707883 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7707883 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7707883 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7707883 | lld:pubmed |
