| pubmed-article:7685022 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7685022 | lifeskim:mentions | umls-concept:C0242772 | lld:lifeskim |
| pubmed-article:7685022 | lifeskim:mentions | umls-concept:C0242724 | lld:lifeskim |
| pubmed-article:7685022 | lifeskim:mentions | umls-concept:C0035685 | lld:lifeskim |
| pubmed-article:7685022 | lifeskim:mentions | umls-concept:C1998468 | lld:lifeskim |
| pubmed-article:7685022 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:7685022 | pubmed:issue | 16 | lld:pubmed |
| pubmed-article:7685022 | pubmed:dateCreated | 1993-7-7 | lld:pubmed |
| pubmed-article:7685022 | pubmed:abstractText | An RNA-directed RNA polymerase (RdRP, EC 2.7.7.48) from tomato leaf tissue was purified to electrophoretic homogeneity. A terminal transferase activity that was found to cofractionate with RdRP from DEAE-Sepharose and DNA-cellulose columns was removed by chromatography on a Mono Q column. The highly purified RdRP exhibits a specific activity of 500 nmol x mg-1 x 30 min-1, which corresponds to a 100,000-fold enrichment of the enzyme. In buffer containing 50% glycerol, its activity decreased by about 15%/month. RdRP activity coincided with the silver staining intensity of a single 128-kDa polypeptide when the fractions eluted from the Mono Q column were analyzed by electrophoresis in a SDS-polyacrylamide gel. Its molecular mass and its sedimentation coefficient of 6.6 S indicate that RdRP is a nearly globular molecule. The catalytic activity of RdRP is resistant to alpha-amanitin and actinomycin D. In tomato leaves systemically infected with potato spindle tuber viroid, the activity of RdRP was found to be increased about 3-fold compared with RdRP isolated from healthy leaf tissue. | lld:pubmed |
| pubmed-article:7685022 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7685022 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7685022 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7685022 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7685022 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7685022 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7685022 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7685022 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:7685022 | pubmed:author | pubmed-author:Marinkovi?SS | lld:pubmed |
| pubmed-article:7685022 | pubmed:author | pubmed-author:HaasBB | lld:pubmed |
| pubmed-article:7685022 | pubmed:author | pubmed-author:SängerH LHL | lld:pubmed |
| pubmed-article:7685022 | pubmed:author | pubmed-author:SchiebelWW | lld:pubmed |
| pubmed-article:7685022 | pubmed:author | pubmed-author:KlannerAA | lld:pubmed |
| pubmed-article:7685022 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7685022 | pubmed:day | 5 | lld:pubmed |
| pubmed-article:7685022 | pubmed:volume | 268 | lld:pubmed |
| pubmed-article:7685022 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7685022 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7685022 | pubmed:pagination | 11851-7 | lld:pubmed |
| pubmed-article:7685022 | pubmed:dateRevised | 2000-12-18 | lld:pubmed |
| pubmed-article:7685022 | pubmed:meshHeading | pubmed-meshheading:7685022-... | lld:pubmed |
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| pubmed-article:7685022 | pubmed:meshHeading | pubmed-meshheading:7685022-... | lld:pubmed |
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| pubmed-article:7685022 | pubmed:meshHeading | pubmed-meshheading:7685022-... | lld:pubmed |
| pubmed-article:7685022 | pubmed:meshHeading | pubmed-meshheading:7685022-... | lld:pubmed |
| pubmed-article:7685022 | pubmed:meshHeading | pubmed-meshheading:7685022-... | lld:pubmed |
| pubmed-article:7685022 | pubmed:meshHeading | pubmed-meshheading:7685022-... | lld:pubmed |
| pubmed-article:7685022 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:7685022 | pubmed:articleTitle | RNA-directed RNA polymerase from tomato leaves. I. Purification and physical properties. | lld:pubmed |
| pubmed-article:7685022 | pubmed:affiliation | Max-Planck-Institut für Biochemie, Martinsried, Germany. | lld:pubmed |
| pubmed-article:7685022 | pubmed:publicationType | Journal Article | lld:pubmed |
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