| pubmed-article:7679924 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C0027754 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C0027752 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C0443286 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C0002198 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C1823153 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C0439855 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C2349976 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C1552644 | lld:lifeskim |
| pubmed-article:7679924 | lifeskim:mentions | umls-concept:C0591833 | lld:lifeskim |
| pubmed-article:7679924 | pubmed:issue | 7 | lld:pubmed |
| pubmed-article:7679924 | pubmed:dateCreated | 1993-3-29 | lld:pubmed |
| pubmed-article:7679924 | pubmed:abstractText | The kallikrein-like serine proteinase nerve growth factor gamma (NGF-gamma) reacted with the plasma proteinase inhibitor human alpha 2-macroglobulin (h alpha 2M). The h alpha 2M subunits were cleaved, the electrophoretic mobility of h alpha 2M in nondenaturing polyacrylamide gels was increased, and the intrinsic fluorescence of h alpha 2M was increased with a slight blue-shift. These changes are well-characterized components of the alpha 2M/proteinase reaction mechanism. In N alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) hydrolysis experiments, the catalytic efficiency (kcat/KM) of the h alpha 2M-NGF-gamma complex was decreased by 98.5% compared with free NGF-gamma. This decrease is unique since other alpha 2M-proteinase complexes retain significant amidase activity. For comparison, we determined that the catalytic efficiency of alpha 2M-trypsin is decreased by 58% compared with free trypsin under equivalent conditions. The rate of NGF-gamma inhibition by h alpha 2M was (1.0 +/- 0.1) x 10(4) M-1 s-1 as determined by BAPNA hydrolysis. A similar value was determined by monitoring the change in intrinsic fluorescence. NGF-gamma, which was bound within the intact 7S NGF complex, also reacted with h alpha 2M, albeit at a very slow rate. This reaction may have depended exclusively on slow reversible dissociation of NGF-gamma from the 7S complex. NGF-gamma was rapidly inhibited by murine alpha 2M (m alpha 2M).(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:7679924 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7679924 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7679924 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7679924 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:7679924 | pubmed:issn | 0006-2960 | lld:pubmed |
| pubmed-article:7679924 | pubmed:author | pubmed-author:GoniasS LSL | lld:pubmed |
| pubmed-article:7679924 | pubmed:author | pubmed-author:VasudevanJJ | lld:pubmed |
| pubmed-article:7679924 | pubmed:author | pubmed-author:WolfB BBB | lld:pubmed |
| pubmed-article:7679924 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7679924 | pubmed:day | 23 | lld:pubmed |
| pubmed-article:7679924 | pubmed:volume | 32 | lld:pubmed |
| pubmed-article:7679924 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7679924 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7679924 | pubmed:pagination | 1875-82 | lld:pubmed |
| pubmed-article:7679924 | pubmed:dateRevised | 2010-10-5 | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:meshHeading | pubmed-meshheading:7679924-... | lld:pubmed |
| pubmed-article:7679924 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:7679924 | pubmed:articleTitle | Reaction of nerve growth factor gamma and 7S nerve growth factor complex with human and murine alpha 2-macroglobulin. | lld:pubmed |
| pubmed-article:7679924 | pubmed:affiliation | Department of Pathology, University of Virginia Health Sciences Center, Charlottesville 22908. | lld:pubmed |
| pubmed-article:7679924 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7679924 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
