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pubmed-article:7671326pubmed:abstractTextChanges in the intracellular Ca2+ levels of human large granular lymphocytes (LGL), loaded with the fluorescent Ca2+ indicator fura-2, have been studied upon addition of human chronic myelogenous leukemia K562 cells. The measurements, analyzed at the single-cell level using image analysis, indicate a rapid Ca2+ mobilization in the effector cell upon interaction with its target cell. This mobilization appeared to be localized to an area within the effector cell that was in physical contact with target cells. The LGL responded with different kinetics in a transient manner and about 19% of them could undergo two or more responses. Data obtained from experiments performed with anti-CD16- and anti-CD18-pretreated LGL in the presence of target cells indicate that the CD16 and CD18 molecules are not likely to be the triggers of the Ca2+ response, although they might participate in the recognition of the target cell.lld:pubmed
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pubmed-article:7671326pubmed:articleTitleRapid Ca2+ mobilization in single LGL cells upon interaction with K562 target cells--role of the CD18 and CD16 molecules.lld:pubmed
pubmed-article:7671326pubmed:affiliationAbo Akademi University, Department of Biochemistry and Pharmacy, Turku, Finland.lld:pubmed
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