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pubmed-article:7663696pubmed:abstractTextA sensitive and reliable analytical procedure has been established for the detection of theophylline (TH), theobromine (TB) and caffeine (CA) in human plasma and urine by gradient capillary high-performance liquid chromatography (HPLC)-frit-fast atom bombardment mass spectrometry (FAB-MS) (LC-frit-FAB-MS). Two capillary columns and a column-switching valve were used in this LC system to allow all of the sample injected to be introduced into the MS system. 7-Ethyltheophylline was used as the internal standard (I.S.). The xanthines in the specimen were extracted with an Extrelut column. The lowest detected amount was ca. 5 ng/ml using this method.lld:pubmed
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pubmed-article:7663696pubmed:dateRevised2011-11-17lld:pubmed
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pubmed-article:7663696pubmed:articleTitleHighly sensitive and rapid determination of theophylline, theobromine and caffeine in human plasma and urine by gradient capillary high-performance liquid chromatography-frit-fast atom bombardment mass spectrometry.lld:pubmed
pubmed-article:7663696pubmed:affiliationDepartment of Forensic Medicine, Fukuoka University School of Medicine, Japan.lld:pubmed
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