| pubmed-article:7641231 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7641231 | lifeskim:mentions | umls-concept:C0006685 | lld:lifeskim |
| pubmed-article:7641231 | lifeskim:mentions | umls-concept:C0079883 | lld:lifeskim |
| pubmed-article:7641231 | lifeskim:mentions | umls-concept:C0392756 | lld:lifeskim |
| pubmed-article:7641231 | lifeskim:mentions | umls-concept:C0521116 | lld:lifeskim |
| pubmed-article:7641231 | lifeskim:mentions | umls-concept:C1879547 | lld:lifeskim |
| pubmed-article:7641231 | lifeskim:mentions | umls-concept:C2346661 | lld:lifeskim |
| pubmed-article:7641231 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:7641231 | pubmed:dateCreated | 1995-9-21 | lld:pubmed |
| pubmed-article:7641231 | pubmed:abstractText | 1. While intracellular calcium concentrations are closely regulated, two types of ion channels in neurons allow calcium influx: both voltage-activated and NMDA-activated channels are significantly permeable to calcium. In this study we compare the effects of lead (Pb2+) on currents carried through voltage-activated calcium channels and NMDA-activated channels. 2. Pb2+ reduces voltage-activated calcium channel currents elicited by a voltage jump from -80 to 0 mV at 0.1 to 1 microM, with an IC50 of 0.64 microM and a Hill slope of 1.22. This effect was partially reversible and not voltage dependent. Sodium and potassium currents were relatively unaffected at Pb2+ concentrations sufficient to block calcium channel currents by more than 80%. Pb2+ is, thus, a potent, reversible and selective blocker of voltage-dependent calcium channel currents. 3. A fast reversible and slow irreversible blocking action of Pb2+ was found on NMDA-activated currents. When Pb2+ was applied simultaneously with aspartate and glycine (Asp/Gly), the inward currents were rapidly and reversibly reduced in a dose-dependent manner with a minimum effective concentration below 2 microM and a total blockade (> 80%) with 100 microM Pb2+. The IC50 was approximately 45 microM and the Hill coefficient 1.1. Preincubation with 50 microM Pb2+ resulted in a greater reduction in the response to Asp/Gly/Pb2+. This effect was reversed within 2 to 5 sec of wash. The lack of voltage dependence suggests that Pb2+ does not block the channel but rather alters the binding of agonists. Prolonged superfusion of a cell with the Asp/Gly/Pb(2+)-containing external solution resulted in a slow and irreversible decrease in the Asp/Gly activated current.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:7641231 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7641231 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7641231 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7641231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7641231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7641231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7641231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7641231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7641231 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7641231 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7641231 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7641231 | pubmed:issn | 0272-4340 | lld:pubmed |
| pubmed-article:7641231 | pubmed:author | pubmed-author:MichaelDD | lld:pubmed |
| pubmed-article:7641231 | pubmed:author | pubmed-author:PlaueRR | lld:pubmed |
| pubmed-article:7641231 | pubmed:author | pubmed-author:BüsselbergDD | lld:pubmed |
| pubmed-article:7641231 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7641231 | pubmed:volume | 14 | lld:pubmed |
| pubmed-article:7641231 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7641231 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7641231 | pubmed:pagination | 711-22 | lld:pubmed |
| pubmed-article:7641231 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7641231 | pubmed:meshHeading | pubmed-meshheading:7641231-... | lld:pubmed |
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| pubmed-article:7641231 | pubmed:meshHeading | pubmed-meshheading:7641231-... | lld:pubmed |
| pubmed-article:7641231 | pubmed:meshHeading | pubmed-meshheading:7641231-... | lld:pubmed |
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| pubmed-article:7641231 | pubmed:meshHeading | pubmed-meshheading:7641231-... | lld:pubmed |
| pubmed-article:7641231 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7641231 | pubmed:articleTitle | Pb2+ reduces voltage- and N-methyl-D-aspartate (NMDA)-activated calcium channel currents. | lld:pubmed |
| pubmed-article:7641231 | pubmed:affiliation | Heinrich-Heine Universität Düsseldorf, Germany. | lld:pubmed |
| pubmed-article:7641231 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7641231 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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