Linked Life Data

7631850

Source:http://linkedlifedata.com/resource/pubmed/id/7631850

Statements in which the resource exists.
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pubmed-article:7631850pubmed:dateCreated1995-9-1lld:pubmed
pubmed-article:7631850pubmed:abstractTextTo investigate the role of the cytoskeleton in cardiac Na+ channel gating, the action of cytochalasin D (Cyto-D), an agent that interferes with actin polymerization, was studied by whole cell voltage clamp and cell-attached and inside-out patches from rat and rabbit ventricular cardiac myocytes. Cyto-D (20-40 microM) reduced whole cell peak Na+ current by 20% within 12 min and slowed current decay without affecting steady-state voltage-dependent availability or recovery from inactivation. Brief treatments (< 10-15 min) of cell-attached patches by Cyto-D (20 microM) in the bath induced short bursts of Na+ channel openings and prolonged decays of ensemble-averaged currents. Bursting of the Na+ channel was more pronounced when the cell suspension was pretreated with Cyto-D (20 microM) for 1 h before seal formation. Application of Cyto-D on the cytoplasmic side of inside-out patches resulted in more dramatic gating changes. Peak open probability was reduced by > 50% within 20 min, and long bursts of openings occurred. Washout of Cyto-D did not restore ensemble-averaged current amplitude, but burst duration decreased toward control values. Cyto-D also induced an additional slower component to open and closed times. These results suggest that Cyto-D, through effects on cytoskeleton, induced cardiac Na+ channels to enter a mode characterized by a lower peak open probability but a greater persistent activity as if the inactivation rate was slowed. The cytoskeleton, in addition to localizing integral membrane proteins, apparently also plays a role in regulating specific detailed functions of integral membrane proteins such as the gating of Na+ channels.lld:pubmed
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pubmed-article:7631850pubmed:dateRevised2007-11-14lld:pubmed
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pubmed-article:7631850pubmed:year1995lld:pubmed
pubmed-article:7631850pubmed:articleTitleCytoskeleton modulates gating of voltage-dependent sodium channel in heart.lld:pubmed
pubmed-article:7631850pubmed:affiliationDivision of Cardiovascular Medicine, Henry Ford Heart and Vascular Institute, Detroit, Michigan 48202, USA.lld:pubmed
pubmed-article:7631850pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:7631850pubmed:publicationTypeResearch Support, U.S. Gov't, P.H.S.lld:pubmed
pubmed-article:7631850pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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