| pubmed-article:7628408 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7628408 | lifeskim:mentions | umls-concept:C0254610 | lld:lifeskim |
| pubmed-article:7628408 | lifeskim:mentions | umls-concept:C0242692 | lld:lifeskim |
| pubmed-article:7628408 | lifeskim:mentions | umls-concept:C0079189 | lld:lifeskim |
| pubmed-article:7628408 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:7628408 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:7628408 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:7628408 | pubmed:dateCreated | 1995-9-1 | lld:pubmed |
| pubmed-article:7628408 | pubmed:abstractText | Interleukin-15 (IL-15) is a recently discovered growth factor which is highly expressed in skeletal muscle. In order to determine a functional role for IL-15 in skeletal myogenesis, the effects of IL-15 on myoblast proliferation and muscle-specific myosin heavy chain (MHC) expression were analyzed using the mouse C2 skeletal myogenic cell line and primary fetal bovine skeletal myogenic cultures. IL-15 had no effect on [3H]thymidine incorporation, nor on the rate of myoblast differentiation, assessed by anti-MHC immunocytochemical staining, in either type of culture. However, Western blot analyses revealed that IL-15 used at concentrations of 10 or 100 ng/ml increased MHC accumulation five-fold in C2 myoblast cultures and 2.5-fold in primary bovine myogenic cultures. Moreover, C2 myotubes formed in the presence of IL-15 appeared larger than controls. These findings indicate IL-15 can stimulate differentiated myocytes and muscle fibers to accumulate increased amounts of contractile proteins. Well-fused primary bovine myogenic cultures treated with the mitotic inhibitor aphidicolin, then administered IL-15 and/or the anabolic growth factor insulin-like growth factor-I (IGF-I), were analyzed for MHC accumulation using Western blots. IL-15 used at 10 ng/ml doubled MHC accumulation and was as effective as IGF-I used at 10 or 100 ng/ml. IL-15 and IGF-I used together increased MHC accumulation close to five-fold, indicating these two factors can act additively on muscle fibers. These findings indicate IL-15 affects parameters associated with skeletal muscle fiber hypertrophy, and suggest that IL-15 may be a novel anabolic agent to increase skeletal muscle mass. | lld:pubmed |
| pubmed-article:7628408 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628408 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7628408 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628408 | pubmed:citationSubset | AIM | lld:pubmed |
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| pubmed-article:7628408 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7628408 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:7628408 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:7628408 | pubmed:author | pubmed-author:GrabsteinK... | lld:pubmed |
| pubmed-article:7628408 | pubmed:author | pubmed-author:QuinnL SLS | lld:pubmed |
| pubmed-article:7628408 | pubmed:author | pubmed-author:HaugkK LKL | lld:pubmed |
| pubmed-article:7628408 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7628408 | pubmed:volume | 136 | lld:pubmed |
| pubmed-article:7628408 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7628408 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7628408 | pubmed:pagination | 3669-72 | lld:pubmed |
| pubmed-article:7628408 | pubmed:dateRevised | 2009-11-19 | lld:pubmed |
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| pubmed-article:7628408 | pubmed:meshHeading | pubmed-meshheading:7628408-... | lld:pubmed |
| pubmed-article:7628408 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7628408 | pubmed:articleTitle | Interleukin-15: a novel anabolic cytokine for skeletal muscle. | lld:pubmed |
| pubmed-article:7628408 | pubmed:affiliation | Geriatric Research, Education, and Clinical Center, American Lake VA Medical Center, Tacoma, WA 98493, USA. | lld:pubmed |
| pubmed-article:7628408 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7628408 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7628408 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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