| pubmed-article:7628375 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7628375 | lifeskim:mentions | umls-concept:C1708248 | lld:lifeskim |
| pubmed-article:7628375 | lifeskim:mentions | umls-concept:C0032005 | lld:lifeskim |
| pubmed-article:7628375 | lifeskim:mentions | umls-concept:C0015283 | lld:lifeskim |
| pubmed-article:7628375 | lifeskim:mentions | umls-concept:C1325847 | lld:lifeskim |
| pubmed-article:7628375 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:7628375 | pubmed:dateCreated | 1995-9-1 | lld:pubmed |
| pubmed-article:7628375 | pubmed:abstractText | Agonist-induced increases in cytoplasmic calcium concentration ([Ca2+]i) play a pivotal role in regulated exocytosis by promoting the fusion of secretory vesicles with the plasma membrane. In permeabilized and ATP-primed pituitary cells, increases in ambient [Ca2+]i stimulated the release of LH from gonadotrophs with an EC50 of 2-3 microM. In contrast, the responses of intact gonadotrophs to agonist stimulation by GnRH were characterized by transient [Ca2+]i elevations of up to 1.5 microM, followed by a plateau of 300-400 nM. The sensitivity of the exocytotic response of permeabilized cells to [Ca2+]i was significantly increased by GnRH, which reduced the EC50 for [Ca2+]i to the submicromolar concentration range. The stimulatory action of GnRH on LH release in permeabilized cells was not a consequence of intracellular Ca2+ release, but was associated with increased cytidine diphosphate diacylglycerol production. Activation of protein kinase C by phorbol esters caused a similar increase in the Ca2+ sensitivity of LH release from permeabilized cells, and this effect was not additive to that of GnRH. Furthermore, the GnRH-induced increase in the sensitivity of the exocytotic response to Ca2+ was attenuated by inhibitors of protein kinase C. These findings indicate that although elevated [Ca2+]i per se can promote LH release from permeabilized gonadotrophs, concomitant activation of protein kinase C is necessary to support exocytosis at the physiological [Ca2+]i levels that prevail in GnRH-stimulated intact cells. Such sensitization of the Ca(2+)-dependent secretory mechanism by protein kinase C may be an important step in the agonist-induced release of LH from pituitary gonadotrophs. | lld:pubmed |
| pubmed-article:7628375 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7628375 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628375 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:7628375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628375 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7628375 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7628375 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:7628375 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:7628375 | pubmed:author | pubmed-author:CattK JKJ | lld:pubmed |
| pubmed-article:7628375 | pubmed:author | pubmed-author:Tomi?MM | lld:pubmed |
| pubmed-article:7628375 | pubmed:author | pubmed-author:ZhengLL | lld:pubmed |
| pubmed-article:7628375 | pubmed:author | pubmed-author:JobinR MRM | lld:pubmed |
| pubmed-article:7628375 | pubmed:author | pubmed-author:StojilkovicS... | lld:pubmed |
| pubmed-article:7628375 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7628375 | pubmed:volume | 136 | lld:pubmed |
| pubmed-article:7628375 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7628375 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7628375 | pubmed:pagination | 3398-405 | lld:pubmed |
| pubmed-article:7628375 | pubmed:dateRevised | 2007-11-15 | lld:pubmed |
| pubmed-article:7628375 | pubmed:meshHeading | pubmed-meshheading:7628375-... | lld:pubmed |
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| pubmed-article:7628375 | pubmed:meshHeading | pubmed-meshheading:7628375-... | lld:pubmed |
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| pubmed-article:7628375 | pubmed:meshHeading | pubmed-meshheading:7628375-... | lld:pubmed |
| pubmed-article:7628375 | pubmed:meshHeading | pubmed-meshheading:7628375-... | lld:pubmed |
| pubmed-article:7628375 | pubmed:meshHeading | pubmed-meshheading:7628375-... | lld:pubmed |
| pubmed-article:7628375 | pubmed:meshHeading | pubmed-meshheading:7628375-... | lld:pubmed |
| pubmed-article:7628375 | pubmed:meshHeading | pubmed-meshheading:7628375-... | lld:pubmed |
| pubmed-article:7628375 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7628375 | pubmed:articleTitle | Gonadotropin-releasing hormone-induced sensitization of calcium-dependent exocytosis in pituitary gonadotrophs. | lld:pubmed |
| pubmed-article:7628375 | pubmed:affiliation | Endocrinology and Reproduction Research Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA. | lld:pubmed |
| pubmed-article:7628375 | pubmed:publicationType | Journal Article | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7628375 | lld:pubmed |
