| pubmed-article:7585542 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C1335858 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C0678222 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C0014939 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C0014912 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C1518174 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C1366536 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C0600508 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7585542 | lifeskim:mentions | umls-concept:C0127400 | lld:lifeskim |
| pubmed-article:7585542 | pubmed:issue | 21 | lld:pubmed |
| pubmed-article:7585542 | pubmed:dateCreated | 1995-12-6 | lld:pubmed |
| pubmed-article:7585542 | pubmed:abstractText | Estrogen receptor (ER)-positive human breast carcinoma (HBC) cell lines express significantly higher levels of retinoic acid receptor alpha (RAR alpha) (isoform 1) mRNA than ER-negative HBCs. Estradiol enhances RAR alpha mRNA expression in different ER-positive HBCs by 2-3-fold, which in turn results in increased sensitivity of ER-positive HBCs to the growth inhibitory effects of retinoic acid. To investigate the regulatory mechanisms of estradiol-mediated enhancement of RAR alpha mRNA expression, the functional promoter for the human RAR alpha isoform 1 was cloned and used to assess estradiol-mediated promoter-dependent enhancement of firefly luciferase reporter gene activity in transiently transfected ER-positive (MCF-7 and T47D) and ER-negative (MDA-MB-231) HBCs. Deletional promoter constructs were obtained to further delineate the promoter region responsible for estradiol-mediated enhancement of promoter activity. Here, we present evidence that approximately 130 bp of the promoter fragment preceding the transcriptional start site are responsible for estradiol-mediated enhancement of hRAR alpha gene expression. The estradiol-mediated enhancement is dependent on ER binding. Further deletional analysis showed that a promoter sequence of 42 base pairs, located approximately 100 bases upstream of the transcriptional start site, contains elements for estradiol-mediated enhancement. Specific deletion of either the Sp1 motif or mutations in the imperfect half-palindromic estrogen response element motif of this fragment abolish its estradiol responsiveness in transient transfections. | lld:pubmed |
| pubmed-article:7585542 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7585542 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7585542 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7585542 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7585542 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7585542 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7585542 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7585542 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7585542 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:KimuraSS | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:BashirelahiNN | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:FontanaJ AJA | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:RishiA KAK | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:SheikhM SMS | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:ShaoZ MZM | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:LiX SXS | lld:pubmed |
| pubmed-article:7585542 | pubmed:author | pubmed-author:BaumannR GRG | lld:pubmed |
| pubmed-article:7585542 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7585542 | pubmed:day | 1 | lld:pubmed |
| pubmed-article:7585542 | pubmed:volume | 55 | lld:pubmed |
| pubmed-article:7585542 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7585542 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7585542 | pubmed:pagination | 4999-5006 | lld:pubmed |
| pubmed-article:7585542 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:7585542 | pubmed:meshHeading | pubmed-meshheading:7585542-... | lld:pubmed |
| pubmed-article:7585542 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7585542 | pubmed:articleTitle | Estradiol regulation of the human retinoic acid receptor alpha gene in human breast carcinoma cells is mediated via an imperfect half-palindromic estrogen response element and Sp1 motifs. | lld:pubmed |
| pubmed-article:7585542 | pubmed:affiliation | Department of Medicine, University of Maryland School of Medicine, Baltimore, USA. | lld:pubmed |
| pubmed-article:7585542 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7585542 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7585542 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
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