| pubmed-article:7580924 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7580924 | lifeskim:mentions | umls-concept:C0020792 | lld:lifeskim |
| pubmed-article:7580924 | lifeskim:mentions | umls-concept:C0206665 | lld:lifeskim |
| pubmed-article:7580924 | lifeskim:mentions | umls-concept:C0015295 | lld:lifeskim |
| pubmed-article:7580924 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:7580924 | pubmed:dateCreated | 1995-12-7 | lld:pubmed |
| pubmed-article:7580924 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7580924 | pubmed:abstractText | We report an extension of 3'-terminal exon trapping technology to the identification of transcribed sequences from yeast artificial chromosomes (YACs). A 350-kb YAC containing mouse genomic DNA was gel-purified and used as the target DNA for the 3'-terminal exon trapping strategy. A novel direct ligation/transfection approach was employed to increase the efficiency of trapping 3'-terminal exons from recombinant vector-derived chimeric mRNA. The resulting RT-PCR product was then used to generate a plasmid library. Randomly chosen individual subclones from this library were sequenced, and the results indicate that 86% met sequence criteria characteristic of 3'-terminal exons, whereas 14% were background from identified sources. PCR mapping efforts suggest eight putative last exons present within this YAC, whereas RT-PCR studies demonstrate that three reside within valid expressed sequences. | lld:pubmed |
| pubmed-article:7580924 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7580924 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7580924 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7580924 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7580924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7580924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7580924 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7580924 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7580924 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:7580924 | pubmed:issn | 1054-9803 | lld:pubmed |
| pubmed-article:7580924 | pubmed:author | pubmed-author:TrentJ MJM | lld:pubmed |
| pubmed-article:7580924 | pubmed:author | pubmed-author:GreenE DED | lld:pubmed |
| pubmed-article:7580924 | pubmed:author | pubmed-author:MeltzerP SPS | lld:pubmed |
| pubmed-article:7580924 | pubmed:author | pubmed-author:KrizmanD BDB | lld:pubmed |
| pubmed-article:7580924 | pubmed:author | pubmed-author:HofmannT ATA | lld:pubmed |
| pubmed-article:7580924 | pubmed:author | pubmed-author:DeSilvaUU | lld:pubmed |
| pubmed-article:7580924 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7580924 | pubmed:volume | 4 | lld:pubmed |
| pubmed-article:7580924 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7580924 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7580924 | pubmed:pagination | 322-6 | lld:pubmed |
| pubmed-article:7580924 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:meshHeading | pubmed-meshheading:7580924-... | lld:pubmed |
| pubmed-article:7580924 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7580924 | pubmed:articleTitle | Identification of 3'-terminal exons from yeast artificial chromosomes. | lld:pubmed |
| pubmed-article:7580924 | pubmed:affiliation | Laboratory of Cancer Genetics, National Center for Human Genome Research, National Institutes of Health, Bethesda, Maryland 20892, USA. | lld:pubmed |
| pubmed-article:7580924 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7580924 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7580924 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7580924 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7580924 | lld:pubmed |
