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| pubmed-article:7568090 | lifeskim:mentions | umls-concept:C0035366 | lld:lifeskim |
| pubmed-article:7568090 | lifeskim:mentions | umls-concept:C0220908 | lld:lifeskim |
| pubmed-article:7568090 | lifeskim:mentions | umls-concept:C1517023 | lld:lifeskim |
| pubmed-article:7568090 | lifeskim:mentions | umls-concept:C1515563 | lld:lifeskim |
| pubmed-article:7568090 | pubmed:issue | 20 | lld:pubmed |
| pubmed-article:7568090 | pubmed:dateCreated | 1995-10-27 | lld:pubmed |
| pubmed-article:7568090 | pubmed:abstractText | Expression cloning of cDNAs was first described a decade ago and was based on transient expression of cDNA libraries in COS cells. In contrast to transient transfection of plasmids, retroviral gene transfer delivers genes stably into a wide range of target cells. We utilize a simple packaging system for production of high-titer retrovirus stock from cDNA libraries to establish a cDNA expression cloning system. In two model experiments, murine interleukin (IL)-3-dependent Ba/F3 cells were infected with libraries of retrovirally expressed cDNA derived from human T-cell mRNA or human IL-3-dependent TF-1 cell line mRNA. These infected Ba/F3 cells were selected for the expression of CD2 by flow cytometry or for the alpha subunit of the human IL-3 receptor (hIL-3R alpha) by factor-dependent growth. CD2 (frequency, 1 in 10(4)) and hIL-3R alpha (frequency, 1 in 1.5 x 10(5)) cDNAs were readily detected in small-scale experiments, indicating this retroviral expression cloning system is efficient enough to clone low-abundance cDNAs by their expression or function. | lld:pubmed |
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| pubmed-article:7568090 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7568090 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7568090 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7568090 | pubmed:month | Sep | lld:pubmed |
| pubmed-article:7568090 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:7568090 | pubmed:author | pubmed-author:KitamuraTT | lld:pubmed |
| pubmed-article:7568090 | pubmed:author | pubmed-author:KinoshitaSS | lld:pubmed |
| pubmed-article:7568090 | pubmed:author | pubmed-author:OnishiMM | lld:pubmed |
| pubmed-article:7568090 | pubmed:author | pubmed-author:ShibuyaAA | lld:pubmed |
| pubmed-article:7568090 | pubmed:author | pubmed-author:MiyajimaAA | lld:pubmed |
| pubmed-article:7568090 | pubmed:author | pubmed-author:NolanG PGP | lld:pubmed |
| pubmed-article:7568090 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7568090 | pubmed:day | 26 | lld:pubmed |
| pubmed-article:7568090 | pubmed:volume | 92 | lld:pubmed |
| pubmed-article:7568090 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7568090 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7568090 | pubmed:pagination | 9146-50 | lld:pubmed |
| pubmed-article:7568090 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7568090 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7568090 | pubmed:articleTitle | Efficient screening of retroviral cDNA expression libraries. | lld:pubmed |
| pubmed-article:7568090 | pubmed:affiliation | Department of Cell Biology, DNAX Research Institute of Molecular and Cell Biology, Palo Alto, CA 94304, USA. | lld:pubmed |
| pubmed-article:7568090 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7568090 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7568090 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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