| pubmed-article:7565758 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C0021757 | lld:lifeskim |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C1417706 | lld:lifeskim |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C2827457 | lld:lifeskim |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C0009017 | lld:lifeskim |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C1336776 | lld:lifeskim |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C0086860 | lld:lifeskim |
| pubmed-article:7565758 | lifeskim:mentions | umls-concept:C1880022 | lld:lifeskim |
| pubmed-article:7565758 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:7565758 | pubmed:dateCreated | 1995-11-21 | lld:pubmed |
| pubmed-article:7565758 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7565758 | pubmed:abstractText | To isolate transcription factors important in the regulation of the human interleukin-3 (IL-3) gene, we screened a lambda gt11 cDNA library, constructed from phytohemagglutinin-stimulated human T-cell RNA, with a probe containing regulatory sequences in the upstream region of the IL-3 gene (located from bp -165 to -128 and referred to as the DNase I footprint A region). We isolated a 0.96-kb cDNA that encoded a basic amino acid domain and a leucine zipper domain and used the "rapid amplification and cloning of 3' ends" technique to isolate the 3' half of the cDNA clone, generating a 1.9-kb full-length cDNA clone. Using in vitro-translated protein, which we call NF-IL3A, we defined the IL-3 promoter sequences bound by NF-IL3A in DNase I footprinting assays as TAATTACGTCTG and, using gel shift assays, defined ATTACG as the minimal sequence required for binding of NF-IL3A in vitro. Proteins that bind to the NF-IL3A binding site are found in both unstimulated and stimulated T-cell lines in similar amounts, although the level of NF-IL3A mRNA increases after T-cell activation in several mature T-cell lines. The NF-IL3A protein is nearly identical to a recently identified transcriptional repressor protein, E4BP4, and NF-IL3A binds specifically to regulatory sequences in both the adenovirus E4 promoter and the human gamma interferon promoter. Cotransfection experiments demonstrate that introduction of an expression vector containing the NF-IL3A cDNA into resting T cells transactivates IL-3 promoter-chloramphenicol acetyltransferase gene plasmids that contain the A region; this effect requires the presence of an intact NF-IL3A binding site. One or more copies of the A region also confer NF-IL3A responsiveness on a heterologous promoter in T cells. NF-IL3A appears to play an important role in the expression of IL-3 by T cells. | lld:pubmed |
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| pubmed-article:7565758 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:7565758 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7565758 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7565758 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7565758 | pubmed:issn | 0270-7306 | lld:pubmed |
| pubmed-article:7565758 | pubmed:author | pubmed-author:FrankRR | lld:pubmed |
| pubmed-article:7565758 | pubmed:author | pubmed-author:KwanKK | lld:pubmed |
| pubmed-article:7565758 | pubmed:author | pubmed-author:ZhangWW | lld:pubmed |
| pubmed-article:7565758 | pubmed:author | pubmed-author:ZhangJJ | lld:pubmed |
| pubmed-article:7565758 | pubmed:author | pubmed-author:NimerS DSD | lld:pubmed |
| pubmed-article:7565758 | pubmed:author | pubmed-author:KornucMM | lld:pubmed |
| pubmed-article:7565758 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7565758 | pubmed:volume | 15 | lld:pubmed |
| pubmed-article:7565758 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7565758 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7565758 | pubmed:pagination | 6055-63 | lld:pubmed |
| pubmed-article:7565758 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7565758 | pubmed:meshHeading | pubmed-meshheading:7565758-... | lld:pubmed |
| pubmed-article:7565758 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7565758 | pubmed:articleTitle | Molecular cloning and characterization of NF-IL3A, a transcriptional activator of the human interleukin-3 promoter. | lld:pubmed |
| pubmed-article:7565758 | pubmed:affiliation | Laboratory of Molecular Aspects of Hematopoiesis, Sloan-Kettering Institute, New York, New York, USA. | lld:pubmed |
| pubmed-article:7565758 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7565758 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7565758 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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