| pubmed-article:7559473 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C0004651 | lld:lifeskim |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C1257994 | lld:lifeskim |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C0376436 | lld:lifeskim |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C1511662 | lld:lifeskim |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C0439605 | lld:lifeskim |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C0205396 | lld:lifeskim |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:7559473 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:7559473 | pubmed:issue | 40 | lld:pubmed |
| pubmed-article:7559473 | pubmed:dateCreated | 1995-11-14 | lld:pubmed |
| pubmed-article:7559473 | pubmed:abstractText | SATB1 is a nuclear matrix attachment DNA (MAR)-binding protein which is predominantly expressed in thymocytes. This protein binds to the minor groove specifically recognizing an unusual DNA context exhibited by a specific MAR region with strong base-unpairing propensity. A phage library displaying nonamer random peptides without any built-in structure was used to identify a MAR binding motif of SATB1. One predominant cyclic peptide C1 of CRQNWGLEGC selected by a MAR-affinity column showed 50% identity with a segment in SATB1 (amino acids 355-363). Replacement of the C1 similarity segment in SATB1 by a random amino acid sequence or its truncation resulted in more than 80% reduction in MAR binding. In contrast, replacement of the same SATB1 segment with the C1 peptide restored full MAR binding activity and specificity as the wild-type protein. Single amino acid mutation of the conserved Arg or Glu residue to Ala greatly reduced MAR binding. Taken together our data show that a nine amino acid sequence in SATB1 represents a key MAR binding motif. Phage display may provide a general tool for rapid identification of DNA binding peptide motifs. | lld:pubmed |
| pubmed-article:7559473 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559473 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559473 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559473 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7559473 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559473 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7559473 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559473 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7559473 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7559473 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7559473 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7559473 | pubmed:issn | 0021-9258 | lld:pubmed |
| pubmed-article:7559473 | pubmed:author | pubmed-author:RuoslahtiEE | lld:pubmed |
| pubmed-article:7559473 | pubmed:author | pubmed-author:Kohwi-Shigema... | lld:pubmed |
| pubmed-article:7559473 | pubmed:author | pubmed-author:KoivunenEE | lld:pubmed |
| pubmed-article:7559473 | pubmed:author | pubmed-author:WandAA | lld:pubmed |
| pubmed-article:7559473 | pubmed:author | pubmed-author:DickinsonL... | lld:pubmed |
| pubmed-article:7559473 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7559473 | pubmed:day | 6 | lld:pubmed |
| pubmed-article:7559473 | pubmed:volume | 270 | lld:pubmed |
| pubmed-article:7559473 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7559473 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7559473 | pubmed:pagination | 23239-42 | lld:pubmed |
| pubmed-article:7559473 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7559473 | pubmed:meshHeading | pubmed-meshheading:7559473-... | lld:pubmed |
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| pubmed-article:7559473 | pubmed:meshHeading | pubmed-meshheading:7559473-... | lld:pubmed |
| pubmed-article:7559473 | pubmed:meshHeading | pubmed-meshheading:7559473-... | lld:pubmed |
| pubmed-article:7559473 | pubmed:meshHeading | pubmed-meshheading:7559473-... | lld:pubmed |
| pubmed-article:7559473 | pubmed:meshHeading | pubmed-meshheading:7559473-... | lld:pubmed |
| pubmed-article:7559473 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7559473 | pubmed:articleTitle | A novel matrix attachment region DNA binding motif identified using a random phage peptide library. | lld:pubmed |
| pubmed-article:7559473 | pubmed:affiliation | Cancer Research Center, La Jolla Cancer Research Foundation, California 92037, USA. | lld:pubmed |
| pubmed-article:7559473 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7559473 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7559473 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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