| pubmed-article:7539886 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C0229535 | lld:lifeskim |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C0019932 | lld:lifeskim |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C0033684 | lld:lifeskim |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C0017337 | lld:lifeskim |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C0076925 | lld:lifeskim |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C0441655 | lld:lifeskim |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C0851285 | lld:lifeskim |
| pubmed-article:7539886 | lifeskim:mentions | umls-concept:C1711351 | lld:lifeskim |
| pubmed-article:7539886 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7539886 | pubmed:dateCreated | 1995-7-10 | lld:pubmed |
| pubmed-article:7539886 | pubmed:abstractText | In an effort to determine whether basic-helix-loop-helix (bHLH) proteins are important in pituitary-specific expression of the alpha-glycoprotein hormone subunit gene, we examined the effect of the dominant negative HLH protein, Id, on the activity of the alpha-subunit gene promoter in pituitary cells. Id over-expression reduces the expression of alpha-subunit reporter genes in either alpha T3-1 gonadotrope-derived or alpha TSH thyrotrope-derived cells. A deletion fragment containing nucleotides from -131 to +44 of the human alpha-subunit promoter is inhibited to a similar degree as a -244 to +44 fragment in alpha T3-1 cells. Nuclear proteins in alpha T3-1 cells bind two potential bHLH protein binding sites (E-boxes, alpha EB1 and alpha EB2) present in this fragment but not to mutations that specifically alter only these sequences. An antibody-specific for upstream stimulatory factor, a widely expressed bHLH-leucine zipper protein, is able to inhibit factor binding to the alpha EB2 sequences but not the alpha EB1 site. Mutating the alpha EB1 element of the alpha-subunit promoter decreases basal activity of this promoter to about 42% of control levels in alpha T3-1 cells. A mutation that abolishes upstream stimulatory factor binding, either alone or in combination with the alpha EB1 mutation, reduces basal activity of the promoter to approximately 21% of control levels in alpha T3-1 cells and abolishes the decrease in promoter activity seen when Id is overexpressed. These results demonstrate that the bHLH family of proteins are important regulators of alpha-subunit gene expression in pituitary cells. | lld:pubmed |
| pubmed-article:7539886 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7539886 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7539886 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7539886 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7539886 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7539886 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7539886 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7539886 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7539886 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7539886 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7539886 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:7539886 | pubmed:issn | 0888-8809 | lld:pubmed |
| pubmed-article:7539886 | pubmed:author | pubmed-author:JacksonS MSM | lld:pubmed |
| pubmed-article:7539886 | pubmed:author | pubmed-author:HoefflerJ PJP | lld:pubmed |
| pubmed-article:7539886 | pubmed:author | pubmed-author:Gutierrez-Har... | lld:pubmed |
| pubmed-article:7539886 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7539886 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:7539886 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7539886 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7539886 | pubmed:pagination | 278-91 | lld:pubmed |
| pubmed-article:7539886 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:7539886 | pubmed:meshHeading | pubmed-meshheading:7539886-... | lld:pubmed |
| pubmed-article:7539886 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7539886 | pubmed:articleTitle | Upstream stimulatory factor, a basic-helix-loop-helix-zipper protein, regulates the activity of the alpha-glycoprotein hormone subunit gene in pituitary cells. | lld:pubmed |
| pubmed-article:7539886 | pubmed:affiliation | Department of Medicine, University of Colorado Health Science Center, Denver 80262, USA. | lld:pubmed |
| pubmed-article:7539886 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7539886 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7539886 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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