| pubmed-article:7534659 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7534659 | lifeskim:mentions | umls-concept:C0035820 | lld:lifeskim |
| pubmed-article:7534659 | lifeskim:mentions | umls-concept:C1135183 | lld:lifeskim |
| pubmed-article:7534659 | lifeskim:mentions | umls-concept:C0205042 | lld:lifeskim |
| pubmed-article:7534659 | lifeskim:mentions | umls-concept:C0035028 | lld:lifeskim |
| pubmed-article:7534659 | lifeskim:mentions | umls-concept:C0009545 | lld:lifeskim |
| pubmed-article:7534659 | lifeskim:mentions | umls-concept:C0205088 | lld:lifeskim |
| pubmed-article:7534659 | lifeskim:mentions | umls-concept:C1517945 | lld:lifeskim |
| pubmed-article:7534659 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:7534659 | pubmed:dateCreated | 1995-4-21 | lld:pubmed |
| pubmed-article:7534659 | pubmed:abstractText | Reperfusion of the ischemic myocardium results in the loss of endothelium-dependent relaxation. We have shown recently that the alternate complement pathway is activated immediately on reperfusion of the ischemic porcine myocardium. We hypothesized that complement activation directly attenuates endothelium-dependent relaxation of porcine coronary arteries. Bradykinin (BK) or substance P concentration-dependently relaxed precontracted (U46619, 50 nmol/L) left anterior descending coronary artery (LAD) rings in vitro. Addition of zymosan to human (10%) or porcine (10%) serum for 30 minutes significantly (P < 0.05) increased the EC50 of BK-induced LAD relaxation from 4 +/- 1 to 418 +/- 159 nmol/L (n = 8) and from 9 +/- 3 to 281 +/- 132 nmol/L (n = 7), respectively. Similarly, addition of zymosan to 10% human serum (HS) for 30 minutes increased the EC50 of substance P-induced LAD relaxation from 0.4 +/- 0.1 to 30 +/- 14 nmol/L (n = 9, P < .05). Basal release of nitric oxide was reduced significantly in LAD rings exposed to zymosan-activated HS compared with HS alone. Addition of soluble CR1 (sCR1, 10 nmol/L) to zymosan-activated HS preserved BK-induced relaxation (EC50) of the LAD rings (control, 4 +/- 1 nmol/L; sCR1 + zymosan+serum, 2 +/- 1 nmol/L; n = 6). Zymosan-activated C8-depleted HS (10%) did not attenuate the EC50 of BK-induced coronary artery relaxation (3 +/- 1 to 3 +/- 1 nmol/L, n = 7, P = NS). Zymosan-activated C8-depleted HS plus C8 (6 micrograms/mL) increased the EC50 of BK-induced coronary artery relaxation from 4 +/- 1 to 423 +/- 141 nmol/L (n = 12, P < .05). We have further demonstrated that C5b-9 complexes can be found on the luminal surface of LAD endothelial cells after 5 minutes of exposure to zymosan-activated HS by using C5b-9 reactive monoclonal antibody fluorescent immunohistochemistry and confocal microscopy.(ABSTRACT TRUNCATED AT 250 WORDS) | lld:pubmed |
| pubmed-article:7534659 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7534659 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7534659 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7534659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7534659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7534659 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7534659 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7534659 | pubmed:month | Apr | lld:pubmed |
| pubmed-article:7534659 | pubmed:issn | 0009-7330 | lld:pubmed |
| pubmed-article:7534659 | pubmed:author | pubmed-author:StahlG LGL | lld:pubmed |
| pubmed-article:7534659 | pubmed:author | pubmed-author:FrendlGG | lld:pubmed |
| pubmed-article:7534659 | pubmed:author | pubmed-author:ReenstraW RWR | lld:pubmed |
| pubmed-article:7534659 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7534659 | pubmed:volume | 76 | lld:pubmed |
| pubmed-article:7534659 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7534659 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7534659 | pubmed:pagination | 575-83 | lld:pubmed |
| pubmed-article:7534659 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:7534659 | pubmed:meshHeading | pubmed-meshheading:7534659-... | lld:pubmed |
| pubmed-article:7534659 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7534659 | pubmed:articleTitle | Complement-mediated loss of endothelium-dependent relaxation of porcine coronary arteries. Role of the terminal membrane attack complex. | lld:pubmed |
| pubmed-article:7534659 | pubmed:affiliation | Brigham and Women's Hospital, Department of Anesthesia, Boston, MA 02115. | lld:pubmed |
| pubmed-article:7534659 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7534659 | pubmed:publicationType | Comparative Study | lld:pubmed |
| pubmed-article:7534659 | pubmed:publicationType | In Vitro | lld:pubmed |
| pubmed-article:7534659 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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