| pubmed-article:7523503 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7523503 | lifeskim:mentions | umls-concept:C0025914 | lld:lifeskim |
| pubmed-article:7523503 | lifeskim:mentions | umls-concept:C0026809 | lld:lifeskim |
| pubmed-article:7523503 | lifeskim:mentions | umls-concept:C0128897 | lld:lifeskim |
| pubmed-article:7523503 | lifeskim:mentions | umls-concept:C0282552 | lld:lifeskim |
| pubmed-article:7523503 | lifeskim:mentions | umls-concept:C0936012 | lld:lifeskim |
| pubmed-article:7523503 | lifeskim:mentions | umls-concept:C0205473 | lld:lifeskim |
| pubmed-article:7523503 | pubmed:issue | 8 | lld:pubmed |
| pubmed-article:7523503 | pubmed:dateCreated | 1994-11-10 | lld:pubmed |
| pubmed-article:7523503 | pubmed:abstractText | Mouse monocyte chemoattractant protein-1 (MCP-1), previously termed JE, is a member of the beta chemokine gene family and a homologue of the human monocyte chemoattractant protein, MCP-1. Mouse rMCP-1 was used to immunize hamsters for the production of mAb. Seven mouse MCP-1-specific mAbs were characterized: two of these mAbs cross-reacted with the human MCP-1, as determined by ELISA. A sensitive and specific capture ELISA for MCP-1 quantitation, which allowed measurement of mouse MCP-1 levels in supernatants from cells stimulated with inflammatory agents, was developed. LPS-stimulated astrocytes produce the highest levels of MCP-1 (80 ng/ml); macrophages and mesangial cells produce lower levels of MCP-1 (2 to 14 ng/ml) after LPS stimulation. IL-1 and TNF-alpha stimulation also can induce low levels of MCP-1 production. Western blot analysis demonstrated that the predominant native form of mouse MCP-1 is a 30-kDa glycoprotein. Two mAbs (2H5 and 6C7) demonstrated dose-dependent neutralization of mouse MCP-1 chemotactic activity. To localize the epitope recognized by one of these neutralizing Abs, the mAb was used to bind a series of genetically engineered truncated variants of human MCP-1. The C-terminal residues 62 to 67 on human MCP-1 molecules seem to be critical to express the epitope recognized by the neutralizing 2H5 anti-MCP-1 mAb. However, multiple sites on the MCP-1 molecule seem to be critical for bioactivity. Thus, these Ab reagents provide a useful tool to explore the biology of the mouse MCP-1 beta chemokine. | lld:pubmed |
| pubmed-article:7523503 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7523503 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7523503 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7523503 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7523503 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:7523503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7523503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7523503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7523503 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7523503 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7523503 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7523503 | pubmed:issn | 0022-1767 | lld:pubmed |
| pubmed-article:7523503 | pubmed:author | pubmed-author:HayashiMM | lld:pubmed |
| pubmed-article:7523503 | pubmed:author | pubmed-author:DorfM EME | lld:pubmed |
| pubmed-article:7523503 | pubmed:author | pubmed-author:LuoYY | lld:pubmed |
| pubmed-article:7523503 | pubmed:author | pubmed-author:LaningJJ | lld:pubmed |
| pubmed-article:7523503 | pubmed:author | pubmed-author:RollinsBB | lld:pubmed |
| pubmed-article:7523503 | pubmed:author | pubmed-author:HancockP RPR | lld:pubmed |
| pubmed-article:7523503 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7523503 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:7523503 | pubmed:volume | 153 | lld:pubmed |
| pubmed-article:7523503 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7523503 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7523503 | pubmed:pagination | 3708-16 | lld:pubmed |
| pubmed-article:7523503 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:meshHeading | pubmed-meshheading:7523503-... | lld:pubmed |
| pubmed-article:7523503 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7523503 | pubmed:articleTitle | Serologic analysis of the mouse beta chemokine JE/monocyte chemoattractant protein-1. | lld:pubmed |
| pubmed-article:7523503 | pubmed:affiliation | Department of Pathology, Harvard Medical School, Boston, MA 02115. | lld:pubmed |
| pubmed-article:7523503 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7523503 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7523503 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7523503 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7523503 | lld:pubmed |
