| pubmed-article:7512496 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0227525 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0282547 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C1149301 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0023636 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0205225 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:7512496 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:7512496 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:7512496 | pubmed:dateCreated | 1994-5-16 | lld:pubmed |
| pubmed-article:7512496 | pubmed:abstractText | In biological fluids, the insulin-like growth factors (IGFs) are associated with binding proteins (IGFBPs), which modify IGF distribution and action. Circulating IGFs are bound predominantly to IGFBP-3, of apparent hepatic origin, but regulation of IGFBP-3 has been difficult to dissect because of the lack of systems suitable for examining hepatic production of IGFBP-3 in vitro. In the present studies, IGFBP-3 expression was identified primarily in hepatic nonparenchymal cells, particularly Kupffer and sinusoidal endothelial cells. Coculture with hepatocytes enhanced the stability of nonparenchymal cells to express IGFBP-3 in vitro. IGFBP-3 in conditioned medium had apparent mol wt of 150-300 kilodaltons, suggesting formation of a ternary complex with IGFs and the acid-labile subunit. Expression and secretion of IGFBP-3 were hormonally responsive and strongly correlated (r = 0.79; P < 0.001), with 2- to 3-fold stimulation by added insulin or IGF-I (both P < 0.05), but not by added GH alone. Our findings suggest that GH may act indirectly to promote IGFBP-3 generation in vivo via increasing both the secretion of insulin and the hepatic production of IGF-I; in patients with diabetes mellitus, reduced circulating levels of IGFBP-3 despite high levels of GH may result from both insulin deficiency and inadequate hepatic production of IGF-I. Coculture of hepatic nonparenchymal and parenchymal cells should be useful for further analysis of the mechanism of IGFBP-3 regulation. | lld:pubmed |
| pubmed-article:7512496 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7512496 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:citationSubset | AIM | lld:pubmed |
| pubmed-article:7512496 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7512496 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7512496 | pubmed:month | May | lld:pubmed |
| pubmed-article:7512496 | pubmed:issn | 0013-7227 | lld:pubmed |
| pubmed-article:7512496 | pubmed:author | pubmed-author:PhillipsL SLS | lld:pubmed |
| pubmed-article:7512496 | pubmed:author | pubmed-author:JL | lld:pubmed |
| pubmed-article:7512496 | pubmed:author | pubmed-author:RamA KAK | lld:pubmed |
| pubmed-article:7512496 | pubmed:author | pubmed-author:VillafuerteB... | lld:pubmed |
| pubmed-article:7512496 | pubmed:author | pubmed-author:KLENAA | lld:pubmed |
| pubmed-article:7512496 | pubmed:author | pubmed-author:BirdsongG GGG | lld:pubmed |
| pubmed-article:7512496 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7512496 | pubmed:volume | 134 | lld:pubmed |
| pubmed-article:7512496 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7512496 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7512496 | pubmed:pagination | 2044-50 | lld:pubmed |
| pubmed-article:7512496 | pubmed:dateRevised | 2011-11-17 | lld:pubmed |
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| pubmed-article:7512496 | pubmed:meshHeading | pubmed-meshheading:7512496-... | lld:pubmed |
| pubmed-article:7512496 | pubmed:meshHeading | pubmed-meshheading:7512496-... | lld:pubmed |
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| pubmed-article:7512496 | pubmed:meshHeading | pubmed-meshheading:7512496-... | lld:pubmed |
| pubmed-article:7512496 | pubmed:year | 1994 | lld:pubmed |
| pubmed-article:7512496 | pubmed:articleTitle | Coculture of primary rat hepatocytes and nonparenchymal cells permits expression of insulin-like growth factor binding protein-3 in vitro. | lld:pubmed |
| pubmed-article:7512496 | pubmed:affiliation | Department of Medicine, Emory University School of Medicine, Atlanta, Georgia 30303. | lld:pubmed |
| pubmed-article:7512496 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7512496 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7512496 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7512496 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:7512496 | lld:pubmed |
