| pubmed-article:7506827 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C1704973 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C0205360 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C0600115 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C0994334 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C0332256 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C2603343 | lld:lifeskim |
| pubmed-article:7506827 | lifeskim:mentions | umls-concept:C1517004 | lld:lifeskim |
| pubmed-article:7506827 | pubmed:issue | 24 | lld:pubmed |
| pubmed-article:7506827 | pubmed:dateCreated | 1994-2-14 | lld:pubmed |
| pubmed-article:7506827 | pubmed:abstractText | UV-absorption spectrophotometry and molecular modeling have been used to study the influence of the chemical nature of sugars (ribose or deoxyribose) on triple helix stability. For the Pyrimidine.purine* Pyrimidine motif, all eight combinations were tested with each of the three strands composed of either DNA or RNA. The chemical nature of sugars has a dramatic influence on triple helix stability. For each double helix composition, a more stable triple helix was formed when the third strand was RNA rather than DNA. No stable triple helix was detected when the polypurine sequence was made of RNA with a third strand made of DNA. Energy minimization studies using the JUMNA program suggested that interactions between the 2'-hydroxyl group of the third strand and the phosphates of the polypurine strand play an important role in determining the relative stabilities of triple-helical structures in which the polypyrimidine third strand is oriented parallel to the polypurine sequence. These interactions are not allowed when the third strand adopts an antiparallel orientation with respect to the target polypurine sequence, as observed when the third strand contains G and A or G and T/U. We show by footprinting and gel retardation experiments that an oligoribonucleotide containing G and A or G and U fails to bind double helical DNA, while the corresponding DNA oligomers form stable triple-helical complexes. | lld:pubmed |
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| pubmed-article:7506827 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7506827 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7506827 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:7506827 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7506827 | pubmed:month | Dec | lld:pubmed |
| pubmed-article:7506827 | pubmed:issn | 0305-1048 | lld:pubmed |
| pubmed-article:7506827 | pubmed:author | pubmed-author:HélèneCC | lld:pubmed |
| pubmed-article:7506827 | pubmed:author | pubmed-author:OttGG | lld:pubmed |
| pubmed-article:7506827 | pubmed:author | pubmed-author:SprinzlMM | lld:pubmed |
| pubmed-article:7506827 | pubmed:author | pubmed-author:GutV AVA | lld:pubmed |
| pubmed-article:7506827 | pubmed:author | pubmed-author:GarestierTT | lld:pubmed |
| pubmed-article:7506827 | pubmed:author | pubmed-author:EscudéCC | lld:pubmed |
| pubmed-article:7506827 | pubmed:author | pubmed-author:FrançoisJ CJC | lld:pubmed |
| pubmed-article:7506827 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7506827 | pubmed:day | 11 | lld:pubmed |
| pubmed-article:7506827 | pubmed:volume | 21 | lld:pubmed |
| pubmed-article:7506827 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7506827 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7506827 | pubmed:pagination | 5547-53 | lld:pubmed |
| pubmed-article:7506827 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:7506827 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:7506827 | pubmed:articleTitle | Stability of triple helices containing RNA and DNA strands: experimental and molecular modeling studies. | lld:pubmed |
| pubmed-article:7506827 | pubmed:affiliation | Laboratoire de Biophysique, Muséum National d'Histoire Naturelle, INSERM U201, CNRS UA481, Paris, France. | lld:pubmed |
| pubmed-article:7506827 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7506827 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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