| pubmed-article:7500991 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C0027923 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C0205147 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C0596698 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C0026882 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C0205359 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C0392762 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C1879746 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C1547011 | lld:lifeskim |
| pubmed-article:7500991 | lifeskim:mentions | umls-concept:C0205556 | lld:lifeskim |
| pubmed-article:7500991 | pubmed:issue | 1-2 | lld:pubmed |
| pubmed-article:7500991 | pubmed:dateCreated | 1996-1-18 | lld:pubmed |
| pubmed-article:7500991 | pubmed:abstractText | The data from forward-mutation experiments to obtain specific-locus mutations at 2 closely linked loci in the adenine-3 (ad-3) region of heterokaryon 12 (H-12) of Neurospora crassa have been tabulated to determine the frequency of spontaneous ad-3 mutations and to determine the percentages resulting from each of the 2 major genotypic classes: gene/point mutations and multilocus deletion mutations. Gene/point mutations at the ad-3B locus (ad-3BR) have been characterized to determine the percentage showing allelic complementation to obtain a presumptive identification of the genetic alteration in each mutation at the molecular level. Data from experiments performed at 2 different laboratories have been compared to assess the interlaboratory reproducibility of quantitative data on H-12. No difference was found between the frequencies of spontaneous specific-locus mutations in the ad-3 region. Genetic analysis of 172 ad-3 mutants demonstrated that specific-locus mutations in the ad-3 region result from both gene/point mutations (82.0% [141/172]), and multilocus deletion mutations (14.5% [25/172]). Heterokaryon tests for allelic complementation demonstrated that 52.5% (53/101) spontaneous ad-3BR mutants show allelic complementation, and result from single base-pair alterations. In addition, 100% (25/25) of the spontaneous multilocus deletion mutations result from the 3 smallest sized genotypic subclasses. The implications of the present experimental data for the validation of the ad-3 specific-locus assay system in Neurospora are discussed. | lld:pubmed |
| pubmed-article:7500991 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7500991 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7500991 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7500991 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7500991 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:7500991 | pubmed:issn | 0027-5107 | lld:pubmed |
| pubmed-article:7500991 | pubmed:author | pubmed-author:MallingH VHV | lld:pubmed |
| pubmed-article:7500991 | pubmed:author | pubmed-author:de SerresF... | lld:pubmed |
| pubmed-article:7500991 | pubmed:author | pubmed-author:BrockmanH EHE | lld:pubmed |
| pubmed-article:7500991 | pubmed:author | pubmed-author:WebberB BBB | lld:pubmed |
| pubmed-article:7500991 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7500991 | pubmed:volume | 332 | lld:pubmed |
| pubmed-article:7500991 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7500991 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7500991 | pubmed:pagination | 45-54 | lld:pubmed |
| pubmed-article:7500991 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7500991 | pubmed:meshHeading | pubmed-meshheading:7500991-... | lld:pubmed |
| pubmed-article:7500991 | pubmed:meshHeading | pubmed-meshheading:7500991-... | lld:pubmed |
| pubmed-article:7500991 | pubmed:meshHeading | pubmed-meshheading:7500991-... | lld:pubmed |
| pubmed-article:7500991 | pubmed:meshHeading | pubmed-meshheading:7500991-... | lld:pubmed |
| pubmed-article:7500991 | pubmed:meshHeading | pubmed-meshheading:7500991-... | lld:pubmed |
| pubmed-article:7500991 | pubmed:meshHeading | pubmed-meshheading:7500991-... | lld:pubmed |
| pubmed-article:7500991 | pubmed:meshHeading | pubmed-meshheading:7500991-... | lld:pubmed |
| pubmed-article:7500991 | pubmed:year | 1995 | lld:pubmed |
| pubmed-article:7500991 | pubmed:articleTitle | Quantitative and qualitative aspects of spontaneous specific-locus mutation in the ad-3 region of heterokaryon 12 of Neurospora crassa. | lld:pubmed |
| pubmed-article:7500991 | pubmed:affiliation | Toxicology Branch, Environmental Toxicology Program, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709-2233, USA. | lld:pubmed |
| pubmed-article:7500991 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7500991 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
