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pubmed-article:7499328pubmed:abstractTextThe Xenopus Y-box protein FRGY2 has a role in the translational silencing of masked maternal mRNA. Here, we determine that FRGY2 will recognize specific RNA sequences. The evolutionarily conserved nucleic acid-binding cold shock domain is required for sequence-specific interactions with RNA. However, RNA binding by FRGY2 is facilitated by N- and C-terminal regions flanking the cold shock domain. The hydrophilic C-terminal tail domain of FRGY2 interacts with RNA independent of the cold shock domain but does not determine sequence specificity. Thus, both sequence-specific and nonspecific RNA recognition domains are contained within the FRGY2 protein.lld:pubmed
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pubmed-article:7499328pubmed:articleTitleSequence-specific RNA recognition by the Xenopus Y-box proteins. An essential role for the cold shock domain.lld:pubmed
pubmed-article:7499328pubmed:affiliationLaboratory of Molecular Embryology, NICHID, National Institutes of Health, Bethesda, Maryland 20892-2710, USA.lld:pubmed
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