| pubmed-article:7487912 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0034721 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0034693 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0009015 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0006556 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0030940 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0030946 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0017262 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0679058 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C1707455 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0679622 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C1547699 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C2700640 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0205314 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C2911684 | lld:lifeskim |
| pubmed-article:7487912 | lifeskim:mentions | umls-concept:C0185117 | lld:lifeskim |
| pubmed-article:7487912 | pubmed:dateCreated | 1995-11-28 | lld:pubmed |
| pubmed-article:7487912 | pubmed:databankReference | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:abstractText | A cDNA encoding a novel rat mast-cell proteinase (MCP) named rMCP-3 was successfully cloned and sequenced from the peritoneal cells of Lewis rats infected with the intestinal nematode Nippostrongylus brasiliensis by using the combination of reverse transcription-PCR and rapid-amplification-of-cDNA-ends ('RACE') methods. The cDNA was 979 bp long and included a 741 bp open reading frame. When the deduced amino acid sequence was compared with those of other known mast-cell proteinases, rMCP-3 was considered to be translated as a preproenzyme with a 19-amino-acid signal peptide, a two-amino-acid activation peptide and a 226-amino-acid mature enzyme. The amino acid identity in the mature enzyme was 52.9% and 55.1% with rMCP-1 and rMCP-2 respectively. The rMCP-3 mRNA was not detected in the peritoneal cells of mast-cell-deficient Ws/Ws rats, though it was strongly detected in those of littermate +/+ and Lewis rats, indicating the mast-cell origin of rMCP-3 In addition to being present in peritoneal mast cells, the rMCP-3 mRNA was strongly detected in the skin, tongue, and RBL2H3 rat basophilic leukaemia cells and weakly in the jejunum of N. brasiliensis-infected rats by RNA blot analysis using a rMCP-3 gene-specific probe. By reverse transcription-PCR, the rMCP-3 mRNA was also detected in the lung. While the expression of rMCP-1 and rMCP-2 are clearly restricted in connective-tissue mast cells and mucosal mast cells respectively, rMCP-3 was widely expressed in both types of mast cells with a predominance in connective-tissue mast cells. | lld:pubmed |
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| pubmed-article:7487912 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7487912 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7487912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7487912 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7487912 | pubmed:month | Oct | lld:pubmed |
| pubmed-article:7487912 | pubmed:issn | 0264-6021 | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:NawaYY | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:KobayashiTT | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:ItohHH | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:MaruyamaHH | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:IdeHH | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:TomitaMM | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:OsadaYY | lld:pubmed |
| pubmed-article:7487912 | pubmed:author | pubmed-author:MurakumoYY | lld:pubmed |
| pubmed-article:7487912 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7487912 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:7487912 | pubmed:volume | 311 ( Pt 2) | lld:pubmed |
| pubmed-article:7487912 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7487912 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7487912 | pubmed:pagination | 675-80 | lld:pubmed |
| pubmed-article:7487912 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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