| pubmed-article:7471102 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7471102 | lifeskim:mentions | umls-concept:C0012854 | lld:lifeskim |
| pubmed-article:7471102 | lifeskim:mentions | umls-concept:C0003241 | lld:lifeskim |
| pubmed-article:7471102 | lifeskim:mentions | umls-concept:C0332157 | lld:lifeskim |
| pubmed-article:7471102 | lifeskim:mentions | umls-concept:C0205470 | lld:lifeskim |
| pubmed-article:7471102 | lifeskim:mentions | umls-concept:C0015111 | lld:lifeskim |
| pubmed-article:7471102 | pubmed:issue | 3 | lld:pubmed |
| pubmed-article:7471102 | pubmed:dateCreated | 1981-5-26 | lld:pubmed |
| pubmed-article:7471102 | pubmed:abstractText | Three immunological methods [radioimmunoassay (RIA), enzyme-linked immunosorbent assay, and radioimmunosorbent technique] were established for quantification of the potentially mutagenic O6-ethyldeoxyguanosine (O6-EtdGuo) in DNA treated with the carcinogen ethylnitrosourea in vivo or in vitro. To obtain high-affinity antibodies for specific detection of low levels of O6-EtdGuo in small amounts of DNA (cells), different schemes were applied for immunization of rabbits with the hapten O6-ethylguanosine coupled to various carrier proteins (rat serum albumin, bovine serum albumin, keyhold limpet hemocyanin). Low-dose immunization with the hapten-keyhold limpet hemocyanin conjugate resulted in antibodies with an affinity constant of 1 to 2 X 10(10) liters/mol and very low levels of cross-reactivity with normal as well as other alkylated DNA components. The RIA (the most sensitive of the three assays) detects 0.05 pmol of O6-EtdGuo at 50% inhibition of tracer (O6-ethyl[8,5'-3H]-3'-deoxyguanosine)-antibody binding. This permits quantification by RIA of O6-EtdGuo at an O6-EtdGuo:2'-deoxyguanosine molar ratio of approximately 3 X 10(-7) in a hydrolysate of 100 micrograms of ethylated DNA. By chromatographic separation of O6-EtdGuo prior to the RIA, this value can be lowered to less than 5 X 10(-8). | lld:pubmed |
| pubmed-article:7471102 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7471102 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7471102 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7471102 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7471102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7471102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7471102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:7471102 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7471102 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7471102 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:7471102 | pubmed:issn | 0008-5472 | lld:pubmed |
| pubmed-article:7471102 | pubmed:author | pubmed-author:MüllerRR | lld:pubmed |
| pubmed-article:7471102 | pubmed:author | pubmed-author:RajewskyM FMF | lld:pubmed |
| pubmed-article:7471102 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7471102 | pubmed:volume | 40 | lld:pubmed |
| pubmed-article:7471102 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7471102 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7471102 | pubmed:pagination | 887-96 | lld:pubmed |
| pubmed-article:7471102 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
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| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:meshHeading | pubmed-meshheading:7471102-... | lld:pubmed |
| pubmed-article:7471102 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:7471102 | pubmed:articleTitle | Immunological quantification by high-affinity antibodies of O6-ethyldeoxyguanosine in DNA exposed to N-ethyl-N-nitrosourea. | lld:pubmed |
| pubmed-article:7471102 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7471102 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:7471102 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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