pubmed-article:7371641 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:7371641 | lifeskim:mentions | umls-concept:C0035668 | lld:lifeskim |
pubmed-article:7371641 | lifeskim:mentions | umls-concept:C0004793 | lld:lifeskim |
pubmed-article:7371641 | lifeskim:mentions | umls-concept:C0028953 | lld:lifeskim |
pubmed-article:7371641 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:7371641 | pubmed:dateCreated | 1980-7-26 | lld:pubmed |
pubmed-article:7371641 | pubmed:abstractText | To study the mechanism of the synthesis of Semliki Forest virus (SFV) 26-S RNA, we have isolated the 5'-terminal 'cap'-containing RNase-T1-resistant oligonucleotide (T1 cap) from the genomic 42-S RNA and from the subgenomic 26-S RNA and determined their nucleotide sequences. The T1 caps were purified from 32P-labelled RNAs on two successive two-dimensional fractionation systems: (a) electrophoresis on cellulose acetate paper followed by homochromatography and (b) two-dimensional polyacrylamide gel electrophoresis. The T1 caps derived from the two RNAs had different mobilities in both systems. Their nucleotide sequence was found to be: m7G(5')ppp-(5')ApUpGp- for the 42-S RNA and m7G(5')ppp(5')ApUpUpGp- for the 26-S RNA, respectively. Thus, it appears that the 26-S RNA is not formed by initiation of the RNA polymerase at the 3' end of the negative-strand template followed by 'cleavage and splicing' or as the result of a 'polymerase jump'. Our results, instead, favour the model of internal initiation of the polymerase on the 42-S negative-strand RNA template. | lld:pubmed |
pubmed-article:7371641 | pubmed:language | eng | lld:pubmed |
pubmed-article:7371641 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7371641 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:7371641 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7371641 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7371641 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7371641 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:7371641 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:7371641 | pubmed:month | Apr | lld:pubmed |
pubmed-article:7371641 | pubmed:issn | 0014-2956 | lld:pubmed |
pubmed-article:7371641 | pubmed:author | pubmed-author:PetterssonR... | lld:pubmed |
pubmed-article:7371641 | pubmed:author | pubmed-author:KääriäinenLL | lld:pubmed |
pubmed-article:7371641 | pubmed:author | pubmed-author:SöderlundHH | lld:pubmed |
pubmed-article:7371641 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:7371641 | pubmed:volume | 105 | lld:pubmed |
pubmed-article:7371641 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:7371641 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:7371641 | pubmed:pagination | 435-43 | lld:pubmed |
pubmed-article:7371641 | pubmed:dateRevised | 2007-7-23 | lld:pubmed |
pubmed-article:7371641 | pubmed:meshHeading | pubmed-meshheading:7371641-... | lld:pubmed |
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pubmed-article:7371641 | pubmed:year | 1980 | lld:pubmed |
pubmed-article:7371641 | pubmed:articleTitle | The nucleotide sequences of the 5'-terminal T1 oligonucleotides of Semliki-Forest-virus 42-S and 26-S RNAs are different. | lld:pubmed |
pubmed-article:7371641 | pubmed:publicationType | Journal Article | lld:pubmed |
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