| pubmed-article:7231436 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:7231436 | lifeskim:mentions | umls-concept:C1882598 | lld:lifeskim |
| pubmed-article:7231436 | lifeskim:mentions | umls-concept:C0034838 | lld:lifeskim |
| pubmed-article:7231436 | lifeskim:mentions | umls-concept:C1521970 | lld:lifeskim |
| pubmed-article:7231436 | pubmed:dateCreated | 1981-7-23 | lld:pubmed |
| pubmed-article:7231436 | pubmed:abstractText | Two biochemical methods are currently available for studying 5-HT receptors in the central nervous system. The first consists of measuring the specific high affinity binding of 3H-5-HT to synaptic membranes. The other derives from the discovery of an adenylate cyclase which can be activated by 5-HT in brain homogenates. Whereas the specific 3H-5-HT binding is measurable in young as well as in adult rats, the 5-HT-sensitive adenylate cyclase can be quantitatively estimated only during the first three weeks following birth. Later on, the increment of adenylate cyclase activity produced by 5-HT is too low to permit valid measurements, notably in tissues from adult rats. Studies on the effects of various agonists and antagonists demonstrated that the specific binding site characterized by a high affinity for 3H-5-HT (Kd = 1.5 nM) exhibited the expected properties of a 5-HT receptor in brain. Performing chemical lesions on serotoninergic neurons by an intracerebral injection of 5, 7-dihydroxytryptamine or the blockade of central 5-HT receptors by the peripheral administration of methiothepin resulted in a subsequent increase in the number of specific binding sites for 3H-5-HT particularly in the hippocampus (+30 to +45%). In contrast, preliminary attempts to detect any supersensitivity of the 5-HT-sensitive adenylate cyclase after selective raphe lesions were unsuccessful. Indeed, several observations strongly suggested that the high affinity binding site for 3H-5-HT did not correspond to the 5-HT receptor coupled to adenylate cyclase in synaptic membranes: 1) the apparent affinity of the 5-HT-sensitive adenylate cyclase for 5-HT was about 300 times lower (Kd = 0.5 microM) than that of the specific 3H-5-HT binding site; 2)the ontogenic evolutions of 3H-5-HT binding and 5-HT-sensitive adenylate cyclase were not parallel, notably in the hippocampus; 3) they were differently affected by several drugs. For instance, quipazine, a putative 5-HT agonist, effectively displaced 3H-5-HT from its specific binding site (Ki = 0.23 microM) whereas it did not affect 5-HT-sensitive adenylate cyclase. In conclusion, it is likely that the high affinity binding site for 3H-5-HT and the 5-HT-sensitive adenylate cyclase belong to two different postsynaptic 5-HT receptors in the rat brain. | lld:pubmed |
| pubmed-article:7231436 | pubmed:language | eng | lld:pubmed |
| pubmed-article:7231436 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7231436 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:7231436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7231436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7231436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7231436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7231436 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:7231436 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:7231436 | pubmed:issn | 0300-5186 | lld:pubmed |
| pubmed-article:7231436 | pubmed:author | pubmed-author:GlowinskiJJ | lld:pubmed |
| pubmed-article:7231436 | pubmed:author | pubmed-author:BockaertJJ | lld:pubmed |
| pubmed-article:7231436 | pubmed:author | pubmed-author:NelsonD LDL | lld:pubmed |
| pubmed-article:7231436 | pubmed:author | pubmed-author:HamonMM | lld:pubmed |
| pubmed-article:7231436 | pubmed:author | pubmed-author:HerbetAA | lld:pubmed |
| pubmed-article:7231436 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:7231436 | pubmed:volume | 7 | lld:pubmed |
| pubmed-article:7231436 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:7231436 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:7231436 | pubmed:pagination | 161-75 | lld:pubmed |
| pubmed-article:7231436 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:7231436 | pubmed:meshHeading | pubmed-meshheading:7231436-... | lld:pubmed |
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| pubmed-article:7231436 | pubmed:meshHeading | pubmed-meshheading:7231436-... | lld:pubmed |
| pubmed-article:7231436 | pubmed:meshHeading | pubmed-meshheading:7231436-... | lld:pubmed |
| pubmed-article:7231436 | pubmed:meshHeading | pubmed-meshheading:7231436-... | lld:pubmed |
| pubmed-article:7231436 | pubmed:year | 1980 | lld:pubmed |
| pubmed-article:7231436 | pubmed:articleTitle | Characteristics of serotonin receptors in the rat brain. | lld:pubmed |
| pubmed-article:7231436 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:7231436 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
